
Full text loading...
Autoradiography of cell envelope ghosts obtained from a strain of Escherichia coli which lacks two major outer membrane proteins has been used to demonstrate the polar concentration of another major outer membrane protein, ompA protein. The β-lactam antibiotic cephalexin prevents the insertion of newly synthesized ompA protein into the poles but removal of the antibiotic allows the randomly dispersed protein to migrate to the polar and possibly the septal areas of the cell. Labelling of whole cells with bacteriophage K3 has confirmed a polar concentration of ompA protein.
Article metrics loading...
Full text loading...
References
Data & Media loading...