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Volume 130, Issue 9

Isolation and Mode of Action of a Staphylococcin-like substance Active Against Gram-positive and Gram-negative Bacteria Free

Abstract

Screening of non-phage group II strains for antagonistic substances revealed one particular strain, D91, to excrete a substance with a wide spectrum of activity; both Gram-positive and Gram-negative bacteria were susceptible. The staphylococcin-like substance D91 produced by this strain was partially purified by column chromatography on Sephadex G-50, DEAE-cellulose, Phenyl Sepharose CL-4B and Sephadex G-200. A molecular weight of 76000 was estimated by gel filtration. The activity was heat sensitive but was not affected by hydrolytic enzymes except for pronase. The protein character of substance D91 was confirmed by gel electrophoresis and subsequent staining with Coomassie blue. The action exerted on sensitive bacteria was bacteriostatic rather than bactericidal. Biosynthesis of DNA, RNA, protein and polysaccharides were inhibited simultaneously in both and Active transport of glutamic acid was stopped in both and , whereas glucose uptake was inhibited in only. The substance induced a slow efflux of Rb from proloaded cells of and The antagonistic activity of D91 was eliminated by ethidium bromide at a rate of 47.6% suggesting that plasmids may be involved in its production.

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© Society for General Microbiology, 1984
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1984-09-01
2023-03-22
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References

  1. Dajani A. S., Wannamaker L. W. 1973a; Kinetic studies on the interaction of bacteriophage type 71 staphylococcal bacteriocin with susceptible bacteria. Journal of Bacteriology 114:738–742
     [Google Scholar]
  2. Dajani A. S., Wannamaker L. W. 1973b; In vitro and in vivo studies on a phage type 7 i staphylococcal bacteriocin. In Staphylococci and Staphylococcal Infection pp. 413–421 Jeljaszewicz J. Edited by Basel: S. Karger;
     [Google Scholar]
  3. Dajani A. S., Taube Z. 1974; Plasmid mediated production of staphylococcin in bacteriophage type 71 Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 5:594–598
     [Google Scholar]
  4. Dajani A. S., Gray E. D. 1970; Effect of bactericidal substance from Staphylococcus aureus on group A streptococci. I. Biochemical alterations. Infection and Immunity 1:485–490
     [Google Scholar]
  5. Dandeu J. P. 1971; Chemical and immunological study of colicins El, K, A, and Q. Infection and Immunity 3:1–9
     [Google Scholar]
  6. Gagliano V. J., Hinsdill R. D. 1970; Characterization of a Staphylococcus aureus bacteriocin. Journal of Bacteriology 104:117–125
     [Google Scholar]
  7. Hale E. M. 1973; Characterization of a bacteriocin from Staphylococcus aureus strain 462. Antimicrobial Agents and Chemotherapy 4:634–640
     [Google Scholar]
  8. Hale E. M., Hinsdill R. D. 1975; Biological activity of staphylococcin 462: bacteriocin from Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 7:74–81
     [Google Scholar]
  9. Herschman H. R., Helinski D. R. 1967; Purification and characterization of colicin E2 and colicin E3. Journal of Biological Chemistry 242:5360–5368
     [Google Scholar]
  10. Jetten A. M., Vogels G. D. 1972a; Nature and properties of a Staphylococcus epidermidis bacteriocin. Journal of Bacteriology 112:243–250
     [Google Scholar]
  11. Jetten A. M., Vogels G. D. 1972b; Mode of action of a Staphylococcus epidermidis bacteriocin. Antimicrobial Agents and Chemotherapy 2:456–463
     [Google Scholar]
  12. Jetten A. M., Vogels G. D., Dewindt F. 1972; Production and purification of a Staphylococcus epidermidis bacteriocin. Journal of Bacteriology 112:235–242
     [Google Scholar]
  13. Konisky J. 1982; Colicins and other bacteriocins with established modes of action. Annual Review of Microbiology 36:125–144
     [Google Scholar]
  14. Kopecky A. L., Copeland D. P., Lusk J. E. 1975; Viability of Escherichia coli treated with colicin K. Proceedings of the National Academy of Sciences of the United States of America 72:4631–4634
     [Google Scholar]
  15. Luria S. E. 1964; On the mechanism of action of colicins. Annales de l’Institut Pasteur 107: suppl. 5 67–73
     [Google Scholar]
  16. Morris D. M., Lawson J. W., Rogolsky M. 1978; Effect of a staphylococcin on Neisseria gonorrhoeae. Antimicrobial Agents and Chemotherapy 14:218–223
     [Google Scholar]
  17. Odeberg H., Olsson I. 1976; Mechanisms for the microbial activity of cationic proteins of human granulocytes. Infection and Immunity 14:1269–1275
     [Google Scholar]
  18. Rogolsky M., Wiley B. B. 1977; Production and properties of staphylococcin genetically controlled by the staphylococcal plasmid for exfoliative toxin synthesis. Infection and Immunity 15:726–732
     [Google Scholar]
  19. Rosen B. P., Kashket E. R. 1982; Energetics of active transport. In Bacterial Transport pp. 559–620 Rosen B. P. Edited by New York & Basel: Marcel Dekker;
     [Google Scholar]
  20. Schein S. J., Kagan B. L., Finkelstein A. 1978; Colicin K acts by forming voltage dependent channels in phospholipid bilayer membranes. Nature; London: 276159–163
     [Google Scholar]
  21. Warren R., Rogolsky M., Wiley B. B., Glasgow L. A. 1974; Effect of ethidium bromide on elimination of exfoliative toxin and bacteriocin production in Staphylococcus aureus. Journal of Bacteriology 118:980–985
     [Google Scholar]
  22. Weerkamp A., Geerts W., Vogels G. D. 1977; Conditional killing effect of staphylococcin and repair of sublethal injury in Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 12:314–321
     [Google Scholar]
  23. Weerkamp A., Heinen-Von Borries U. J., Vogels G. D. 1978; Biochemical and ultrastructural changes in Staphylococcus aureus treated with staphylococcin 1580. Antonie van Leeuwenhoek 44:35–48
     [Google Scholar]
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Isolation and Mode of Action of a Staphylococcin-like substance Active Against Gram-positive and Gram-negative Bacteria
Microbiology 130, 2291 (1984); https://doi.org/10.1099/00221287-130-9-2291
/content/journal/micro/10.1099/00221287-130-9-2291
/content/journal/micro/10.1099/00221287-130-9-2291
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