1887

Abstract

Two murine monoclonal antibodies of the IgG3 class have been isolated after immunization with An indirect immunofluorescence test was used to screen hybridoma supernatants and subsequently to determine the cross-reactivity of the monoclonal antibodies with other bacteria. One monoclonal antibody reacted with all the smooth biotypes tried and with serogroup O:9, though not with rough or with strains of and The other monoclonal antibody displayed a high degree of specificity for brucellae carrying the A lipopolysaccharide-protein surface antigen. The implications for the diagnosis of brucellosis are discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-9-2285
1984-09-01
2021-08-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/9/mic-130-9-2285.html?itemId=/content/journal/micro/10.1099/00221287-130-9-2285&mimeType=html&fmt=ahah

References

  1. Ahvonen P., Jansson E., Aho K. 1969; Marked cross-agglutination between Brucellae and a sub-type of Yersinia enterocolitica. Acta pathologica et microbiologica scandinavica 75:291–295
    [Google Scholar]
  2. Biegeleisen J. Z., Moody M. D. 1960; Genus specificity of fluorescein-labelled anti -Brucellameli- tensis globulin. Bacteriological Proceedings140–141
    [Google Scholar]
  3. Biegeleisen J. Z., Bradshaw B. R., Moody M. D. 1962; Demonstration of Brucella antibodies in human serum. Journal of Immunology 88:109–112
    [Google Scholar]
  4. Brinley-Morgan W. J., Mccullough N. B. 1974; Genus Brucella Meyer and Shaw 1920, 173. In Bergey’s Manual of Determinative Bacteriology, 8th edn. pp. 278–282 Buchanan R. E., Gibbons N. E. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  5. Cioglia L. 1950; Antigenicommuni a brucelle e salmonelle. Giomale di batteriologia e immunologia 42:81–90
    [Google Scholar]
  6. Corbel M. J. 1973; The direct fluorescent antibody test for detection of Brucella abortus in bovine abortion material. Journal of Hygiene 71:123–129
    [Google Scholar]
  7. Corbel M. J. 1975; The serological relationship between Brucella spp., Yersinia enterocolitica serotype IX and Salmonella serotypes of Kauffmann-White group N. Journal of Hygiene 75:151–171
    [Google Scholar]
  8. Corbel M. J., Day C. A. 1973; Assessment of fluorescent antibody absorption procedures for differentiation of the serological response to Yersinia enterocolitica serotype IX and Brucella abortus in cattle. British Veterinary Journal 129:lxvii–lxxi
    [Google Scholar]
  9. Diaz R., Bosseray N. 1974; Estudio de las relaciones antigénicas entre Y. enterocoliticaserotipo 9 y otras especias bacterianas gram-negatives. Microbiologia espanola 27:1–14
    [Google Scholar]
  10. Diaz R., Jones L. M., Wilson J. B. 1968a; Antigenic relationship of the Gram negative organism causing canine abortion to smooth and rough brucellae. Journal of Bacteriology 95:618–624
    [Google Scholar]
  11. Diaz R., Jones L. M., Leong D., Wilson J. B. 1968b; Surface antigens of smooth brucellae. Journal of Bacteriology 96:893–901
    [Google Scholar]
  12. Diaz R., Lacalle R., Medrano P., Leong D. 1970; Immunobiological activities of the endotoxin of Yersinia enterocolitica, type MY79. Proceedings of the Vth International Congress on Infectious Diseases, Vienna; bacteria 2:11–17
    [Google Scholar]
  13. Kohler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature; London: 296495–497
    [Google Scholar]
  14. Moody M. D., Biegeleisen J. Z., Taylor G. C. 1961; Detection of brucellae and their antibodies by fluorescent antibody and agglutination tests. Journal of Bacteriology 81:990–995
    [Google Scholar]
  15. Morse S. I., Morse J. H. 1976; Isolation and properties of the leucocytosis and lymphocytosis promoting factor of Bordetella pertussis. Journal of Experimental Medicine 143:1483–1502
    [Google Scholar]
  16. Phillips A. P., Martin K. L. 1982a; Evaluation of a microfluorometer in immunofluorescence assays of individual spores of Bacillus anthracis and Bacillus cereus. Journal of Immunological Methods 49:271–282
    [Google Scholar]
  17. Phillips A. P., Martin K. L. 1982b; Assessment of immunofluoresence measurements of individual bacteria in direct and indirect assays for Bacillus anthracis and Bacillus cereus spores. Journal of Applied Bacteriology 53:223–231
    [Google Scholar]
  18. Raybould T. J. G. 1982; Antigens of diagnostic significance in Brucella abortus. Canadian Journal of Microbiology 28:557–566
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-9-2285
Loading
/content/journal/micro/10.1099/00221287-130-9-2285
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error