1887

Abstract

Hybridization of eight species of the group was attempted using auxotrophic mutants and protoplast fusion methods. Viable fusion products were obtained from eight crosses. Allodiploid hybrids were recovered from crosses involving with var. and , although some mutants only gave heterokaryons. Crosses involving these latter species also gave heterokaryons. Crosses between and and were unsuccessful. Fusions involving three parents gave heterokaryons made up of only two of them.

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1984-09-01
2024-12-06
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References

  1. Anné J., Eyssen H. 1978; Isolation of interspecies hybrids of Penicillium citrinum and Penicillium cyarteo-fulvaum following protoplast fusion. FEMS Microbiology Letters 4:87–90
    [Google Scholar]
  2. Anné J., Peberdy J. F. 1975; Conditions for induced fusion of fungal protoplasts in polyethylene glycol solutions. Archives of Microbiology 105:201–205
    [Google Scholar]
  3. Anné J., Peberdy J. F. 1976; Induced fusion of fungal protoplasts following treatment with polyethylene glycol. Journal of General Microbiology 92:413–417
    [Google Scholar]
  4. Anné J., Eyssen H., Desomer P. 1976; Somatic hybridization of Penicillium roqueforti with Penicillium chrysogenum after protoplast fusion. Nature; London: 262:719–721
    [Google Scholar]
  5. Bradshaw R. E., Lee K.-U., Peberdy J. F. 1983; Aspects of genetic interaction in hybrids of Aspergillus nidulans and Aspergillus rugulosus obtained by protoplast fusion. Journal of General Microbiology 129:3525–3533
    [Google Scholar]
  6. Catcheside D. C. 1954; Isolation of nutritional mutants of Neurospora crassa by filtration enrichment. Journal of General Microbiology 11:34–36
    [Google Scholar]
  7. Clutterbuck A. J. 1973; Gene symbols in Aspergillus nidulans. Genetical Research 21:291–296
    [Google Scholar]
  8. Croft J. H. 1985; Protoplast fusion and incompatibility in Aspergillus. In Fungal Protoplasts: Uses in Biochemistry and Genetics. Peberdy J. F., Ferenczy L. Edited by New York: Marcel Dekker; (in the Press)
    [Google Scholar]
  9. Croft J. H., Jinks J. L. 1977; Aspects of population genetics of Aspergillus nidulans. In Genetics and Physiology of Aspergillus pp. 339–360 Smith J. E., Pateman J. A. Edited by London, New York: Academic Press;
    [Google Scholar]
  10. Dales R. B. G, Turner G. 1980; The transfer of mitochondria between species of Aspergillus. In Advances in Protoplast Research pp. 85–92 Ferenczy L., Farkas G. L. Edited by Budapest: Akademiai Kiado Oxford:: Pergamon Pressr;
    [Google Scholar]
  11. Dales R. B. G. 1978 Heterokaryon incompatibility in Aspergillus nidulans. PhD thesis. University of Birmingham; UK:
    [Google Scholar]
  12. Dales R. B. G, Croft J. H. 1977; Protoplast fusion and isolation of heterokaryons and diploids from vegetatively incompatible strains of Aspergillus nidulans. FEMS Microbiology Letters 1:201–203
    [Google Scholar]
  13. Dales R. B. G, Croft J. H. 1980; Protoplast fusion and the genetical analysis of vegetative incompatibility in Aspergillus nidulans. In Advances in Protoplast Research pp. 73–84 Ferenczy L., Farkas G. L. Edited by Budapest: Akademiai Kiado. Oxford: Pergamon Press;
    [Google Scholar]
  14. Ferenczy L. 1976; Some characteristics of intra- and interspecific protoplast fusion products of Aspergillus nidulans and Aspergillus fumigatus. In Cell Genetics in Higher Plants pp. 171–183 Dudits D., Farkas G. L., Maliga P. Edited by Budapest: Akademiai Kiado. Oxford: Pergamon Press;
    [Google Scholar]
  15. Ferenczy L., Kevei F., Szegedi M. 1975a; Increased fusion frequency of Aspergillus nidulans protoplasts. Experientia 31:50–52
    [Google Scholar]
  16. Ferenczy L"KEVEI, Szegedi M. 1975b; High- frequency fusion of fungal protoplasts. Experientia 31:1028–1030
    [Google Scholar]
  17. Ferenczy L., Kevei F., Szegedi M., Frankó A., Rojik I. 1976; Factors affecting high frequency fungal protoplast fusion. Experientia 32:1156–1158
    [Google Scholar]
  18. Ferenczy L., Szegedi M., Kevei F. 1977; Interspecific protoplast fusion and complementation in aspergilli. Experientia 33:184–185
    [Google Scholar]
  19. Kevei F., Peberdy J. F. 1977; Interspecific hybridization between Aspergillus nidulans and Aspergillus rugulosus by fusion of somatic protoplasts. Journal of General Microbiology 102:255–262
    [Google Scholar]
  20. Kevei F., Peberdy J. F. 1979; Induced segregation in interspecific hybrids of Aspergillus nidulans and Aspergillus rugulosus obtained by protoplast fusion. Molecular and General Genetics 170:213–218
    [Google Scholar]
  21. Kevei F., Peberdy J. F. 1985; Interspecific hybridisation following protoplast fusion, in Aspergillus. In Fungal Protoplasts: Uses in Biochemistry and Genetics. Peberdy J. F., Ferenczy L. Edited by New York: Marcel Dekker; (in the Press
    [Google Scholar]
  22. Mccully K. S., Forbes E. 1965; The use of p- fluorophenylalanine wjth ‘master strains’ of Aspergillus nidulans for assigning genes to linkage groups. Genetical Research 6:352–359
    [Google Scholar]
  23. Peberdy J. F., Isaac S. 1976; An improved procedure for the isolation of protoplasts from Aspergillus nidulans. Microbios Letters 3:7–9
    [Google Scholar]
  24. Peberdy J. F., Eyssen H., Anné J. 1977; Interspecific hybridization between Penicillium chrysogenum and Penicillium cyaneo-fulvum following protoplast fusion. Molecular and General Genetics 157:281–284
    [Google Scholar]
  25. Pontecorvo G., Roper J. A., Hemmons L. M., Macdonald K. D., Bufton A.W.J. 1953; The genetics of Aspergillus nidulans. Advances in Genetics 5:141–238
    [Google Scholar]
  26. Raper K. B., Fennell D. I. 1965 The Genus Aspergillus. Baltimore; Williams and Wilkins:
    [Google Scholar]
  27. Slater M. L. 1976; Rapid nuclear staining method for Saccharomyces cerevisiae. Journal of Bacteriology 126:1339–1341
    [Google Scholar]
  28. Upshall A., Giddings B., Mortimore D. 1977; The use of benlate for distinguishing between haploid and diploid strains of Aspergillus nidulans and Aspergillus terreus. Journal of General Microbiology 100:413–418
    [Google Scholar]
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