1887

Abstract

-Acetylglucosaminidase was induced by either -acetylglucosamine or -acetylmannosamine in several strains of . Enzyme activity was not induced in a -acetylglucosamine non-utilizing mutant which is unable to express the first three steps in the -acetylglucosamine catabolic pathway. The enzyme, purified 500-fold, had a specific activity of 36·8 units (mg protein) and catalysed the hydrolysis of -nitrophenyl---acetylglucosamine, -diacetylchitobiose and -triacetylchitotriose. No activity was observed toward colloidal chitin, hyaluronic acid or mucin. The cellular distribution of -acetylglucosaminidase was determined by measuring enzyme activity before and after acid treatment of intact cells. -Acetylglucosaminidase (80–88% of the total cellular activity) was rapidly secreted to the periplasm when the enzyme was induced either during yeast growth at 28 °C or germ-tube formation at 37 °C. Export of the enzyme from the periplasm into the medium was fourfold greater during germ-tube formation, and after 6 h incubation the amount of enzyme released into the medium represented 70% of cell-associated enzyme activity.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-9-2213
1984-09-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/9/mic-130-9-2213.html?itemId=/content/journal/micro/10.1099/00221287-130-9-2213&mimeType=html&fmt=ahah

References

  1. Bradford M. M. 1976; A rapid and sensitive method for quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  2. Cassone A., Simonetti N., Strippoli V. 1973; Ultrastructural changes in the wall during germ-tube formation from blastospores of Candida albicans. Journal of General Microbiology 77:417–426
    [Google Scholar]
  3. Eggstein M. 1967; In Techniques in Protein Chemistry. p. 340 Bailey J. L. Edited by London: Elsevier;
    [Google Scholar]
  4. Gopal P., Sullivan P. A. 1982; Enzymes of N-acetylglucosamine metabolism during germ-tube formation in Candida albicans. Journal of General Microbiology 128:2319–2326
    [Google Scholar]
  5. Jeuniaux C. 1966; Chitinases. Methods in Enzymo-logy 8:644–650
    [Google Scholar]
  6. Macdonald F., Odds F. C. 1983; Virulence for mice of a proteinase-secreting strain of Candida albicans and a proteinase-deficient mutant. Journal of General Microbiology 129:431–438
    [Google Scholar]
  7. Odds F.C. 1979 Candida and Candidosis pp. 29–41 Leicester: Leicester University Press;
    [Google Scholar]
  8. Pugh D., CAWSON R. A. 1977; The cytochemical localization of acid hydrolases in four common fungi. Cellular and Molecular Biology 22:125–132
    [Google Scholar]
  9. Ram S. P., Sullivan P. A., Shepherd M. G. 1983; The in situ assay of Candida albicans enzymes during yeast growth and germ-tube formation. Journal of General Microbiology 129:2367–2378
    [Google Scholar]
  10. Ram S. P., Romana L. K., Shepherd M. G., Sullivan P. A. 1984; Exo-(l→3)-β-glucanase, autolysin and trehalase activities during yeast growth and germ-tube formation in Candida albicans. Journal of General Microbiology 130:1227–1236
    [Google Scholar]
  11. Reissig J. L., Strominger J. L., Leloir L. F. 1955; A modified colorimetric method for the estimation ofN-acetylamino sugars. Journal of Biological Chemistry 217:959–966
    [Google Scholar]
  12. Shepherd M. G., Sullivan P. A. 1976; The production and growth characteristics of yeast and mycelial forms of Candida albicans in continuous culture. Journal of General Microbiology 93:361–370
    [Google Scholar]
  13. Shepherd M. G., Sullivan P. A. 1983; Candida albicans germ-tube formation with immobilized GlcNAc. FEMS Microbiology Letters 17:167–170
    [Google Scholar]
  14. Shepherd M. G., Chiew Y. Y., Ram S. P. 1980a; Germ-tube induction in Candida albicans. Canadian Journal of Microbiology 26:21–26
    [Google Scholar]
  15. Shepherd M. G., Ghazali H. M., Sullivan P. A. 1980b; N-Acetyl-d-glucosamine kinase and germ- tube formation in Candida albicans. Experimental Mycology 4:147–159
    [Google Scholar]
  16. Simonetti N., Strippoli V., Cassone A. 1974; Yeast-mycelial conversion induced by N-acetyl-d-glucosamine in Candida albicans. Nature; London: 250344–346
    [Google Scholar]
  17. Sullivan P. A., Shepherd M. G. 1982; Gratuitous induction by N-acetylmannosamine of germtube formation and enzymes for N-acetyl-glucosamine utilisation in Candida albicans. Journal of Bacteriology 151:1118–1122
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-9-2213
Loading
/content/journal/micro/10.1099/00221287-130-9-2213
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error