1887

Abstract

The Calvin cycle enzyme phosphoribulokinase has been localized in terms of catalytic activity and enzyme protein in the cyanobacterium In contrast to the CO-fixing enzyme of the Calvin cycle, -ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), which occurs in the cytoplasm and in the carboxysomes, phosphoribulokinase is essentially (95%) cytoplasmic in extracts from cells grown photoautotrophically to late exponential phase. Total phosphoribulokinase accounted for 0·6% of total cell protein in these cells. Immunochemical identity was found between the phosphoribulokinase located in the cytoplasm and the remaining enzyme (5%) associated with the particulate fraction of the cell obtained following differential centrifugation. On density gradient centrifugation of the particulate fraction into Percoll plus sucrose the RuBisCO activity was located in a carboxysome band in the lower half of the gradient while the phosphoribulokinase activity essentially remained concentrated at the top of the gradient. Comparison of RuBisCO and phosphoribulokinase distributions suggests that the latter is not a carboxysomal enzyme in late exponential phase cells of .

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/content/journal/micro/10.1099/00221287-130-8-2089
1984-08-01
2024-04-25
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References

  1. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  2. Beudeker R. F., Kuenen J. G. 1981; Carboxysomes: ‘Calvinosomes’?. FEBS Letters 131:269–274
    [Google Scholar]
  3. Beudeker R. F., Codd G. A., Kuenen J. G. 1981; Quantification and intracellular distribution of ribulose 1,5-bisphosphate carboxylase in Thio-bacillus neapolitanus, as related to possible functions of carboxysomes. Archives of Microbiology 129:361–367
    [Google Scholar]
  4. Biedermann M., Westphal K. 1979; Chemical composition of NB1 particles from Nitrobacter agilis. . Archives of Microbiology 121:187–191
    [Google Scholar]
  5. Cannon G. C., Shively J. M. 1983; Characterization of a homogeneous preparation of carboxysomes from Thiobacillus neapolitanus. . Archives of Microbiology 134:52–59
    [Google Scholar]
  6. Codd G. A., Stewart W. D. P. 1973; Pathways of glycollate metabolism in the blue-green alga Ana-baena cylindrica. . Archiv für Mikrobiologie 94:11–28
    [Google Scholar]
  7. Codd G. A., Stewart W. D. P. 1976; Polyhedral bodies and ribulose 1,5-diphosphate carboxylase of the blue-green alga Anabaena cylindrica. . Planta 130:323–326
    [Google Scholar]
  8. Harms H., Koops H.-P., Martiny H., Wullen-Weber M. 1981; d-Ribulose 1,5-bisphosphate carboxylase and polyhedral inclusion bodies in Nitrosomonas spec. Archives of Microbiology 128:280–281
    [Google Scholar]
  9. Lanaras T., Codd G. A. 1981a; Ribulose 1,5-bisphosphate carboxylase and polyhedral bodies of Chlorogloeopsis fritschii. . Planta 153:279–285
    [Google Scholar]
  10. Lanaras T., Codd G. A. 1981b; Structural and immunoelectrophoretic comparison of soluble and particulate ribulose bisphosphate carboxylases from the cyanobacterium Chlorogloeopsis fritschii. . Archives of Microbiology 130:213–217
    [Google Scholar]
  11. Lanaras T., Codd G. A. 1982; Variations in ribulose 1,5-bisphosphate carboxylase protein levels, activities and subcellular distribution during photoautotrophic batch culture of Chlorogloeopsis fritschii. . Planta 154:284–288
    [Google Scholar]
  12. Leadbeater L., Siebert K., Schobert P., Bowien B. 1982; Relationship between activities and protein levels of ribulose-bisphosphate carboxylase and phosphoribulokinase in Alcaligenes eutrophus. . FEMS Microbiology Letters 14:263–266
    [Google Scholar]
  13. Marsden W. J. N, Codd G. A. 1984; Purification and molecular and catalytic properties of phosphoribulokinase from the cyanobacterium Chlorogloeopsis fritschii. . Journal of General Microbiology 130:999–1006
    [Google Scholar]
  14. Shively J. M., Ball F. L., Brown D. H., Saunders R. E. 1973; Functional organelles in prokaryotes: polyhedral bodies (carboxysomes) of Thiobacillus neapolitanus. . Science 182:584–586
    [Google Scholar]
  15. Shively J. M., Bock E., Westphal K., Cannon G. C. 1977; Icosahedral inclusions (carboxysomes) of Nitrobacter agilis. . Journal of Bacteriology 132:673–675
    [Google Scholar]
  16. Stanier R. Y., Kunisawa R., Mandel M., Cohen-Bazire G. 1971; Purification and properties of unicellular blue-green algae (order Chroococcales). . Bacteriological Reviews 35:171–205
    [Google Scholar]
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