As the sole source of nitrogen, methylamine supported the growth of a range of species of The methylamine assimilatory system was inducible in MNF3841, whereas the capacity to utilize NH as a nitrogen source was constitutive. An uptake system for [C]methylamine (methylamine permease) was induced by growth of MNF3841 on methylamine or ethylamine. The uptake was sensitive to 2,4-dinitrophenol, azide and carbonyl cyanide -chlorophenylhydrazone. The methylamine permease had a of 0.035 mM, a of 2.2 nmol min (mg protein) and a for ammonium of 1.5 mM. Most of the [C]methylamine accumulated by cells was rapidly incorporated into TCA-insoluble materials. An NH -sensitive methylamine-accumulating system distinct from the methylamine permease was demonstrated in ammonia-limited cells grown in continuous culture. This system, the ammonium permease, had a of 0.11 mM (for methylamine), a for NH of 0.007 mM and a , of 2.5 nmol min (mg protein). Methylamine was accumulated by chemostat-grown, N-limited cells and could exchange with unlabelled methylamine. Treatment with carbonyl cyanide -chlorophenylhydrazone caused efflux of the accumulated methylamine, whereas high concentrations of NH did not. Thus possesses a specific methylamine permease which is quite distinct from the ammonium permease.


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