Deep-sea bacteria were isolated from the digestive tract of animals inhabiting depths of 5900 m in the Puerto Rico Trench and 4300 m near the Walvis Ridge. Growth of two bacterial strains was measured in marine broth and in solid media under a range of pressures and temperatures. Both strains were barophilic at 2 °C (±1 °C) with an optimal growth rate of 0.22 h at a pressure 30% lower than that encountered At 1 atm they grew at temperatures ranging from 1.2 to 18.2 °C (±0.3 °C), while pressures increased the upper temperature limit to 23.3 °C. Both strains were identified as members of the genus , based on standard taxonomic tests and mol % G + C values (47.0 and 47.1). Ribonucleotide sequences determined for 5S ribosomal RNA from each strain confirmed relationship to the group, as represented by and , but the barophiles were clearly distinct from these species. Secondary structure conformed to the established model for eubacterial 5S rRNA.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error