1887

Microbiology Society logo

Volume 130, Issue 7

The Acid End-products of Glucose Metabolism of Oral and Other Haemophili Free

Abstract

The acids produced in broth culture by various species of oral haemophili and by stock strains of capsulated and other haemophili were identified and measured by gas-liquid chromatography. Succinic acid was the major acid end-product of all strains, with acetic acid also being regularly produced but in smaller amounts. A stock strain, NCTC 4101, produced less succinic acid than other strains of this species and produced much more oxalacetic and pyruvic acid than all the other strains of haemophili. Strain NCTC 4101 possessed all the enzymes of the tricarboxylic acid cycle, as previously reported, but in the other haemophili examined only succinic dehydrogenase, fumarase and malate dehydrogenase could be detected. No other enzymes of the tricarboxylic acid cycle were detected and isocitrate lyase, malate synthase and pyruvate carboxylase were also absent. Phosphoenolpyruvate-carboxylase was present in all strains. A partial tricarboxylic acid cycle and marked malate dehydrogenase activity appear to be characteristic of haemophili. The pathway to succinate in haemophili appears to be via carboxylation of phosphoenolpyruvate to oxalacetate and thence via malate and fumarate. The results of tracer studies on a single oral strain of using various labelled substrates were in keeping with this proposed metabolic pathway.

  • Received:
  • Revised:
  • Published Online:
© Society for General Microbiology 1984
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-7-1787
1984-07-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/7/mic-130-7-1787.html?itemId=/content/journal/micro/10.1099/00221287-130-7-1787&mimeType=html&fmt=ahah

References

  1. Hofstad T. 1974; The distribution of heptose and 2- keto-3-deoxyoctonate in Bacteriodaciae. Journal of General Microbiology 85:314–320
     [Google Scholar]
  2. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe Laboratory Manual, 4th edn.. pp. 30–38134–140 Blacksburg: VPI Anaerobe Laboratory;
     [Google Scholar]
  3. Hollander R. 1976; Energy metabolism of some representatives of the Haemophilus group. Antonie van Leeuwenhoek 42:429–444
     [Google Scholar]
  4. Jurtshuk P., May A. K., Pope L. M., Aston P. R. 1969; Comparative studies on succinate and terminal oxidase activity in microbial electron transport system. Canadian Journal of Microbiology 15:797–807
     [Google Scholar]
  5. Kilian M. 1976; A taxonomic study of the genus Haemophilus, with the proposal of a new species. Journal of General Microbiology 93:9–62
     [Google Scholar]
  6. Kröger A., Dadak V., Klingenberg M., Deimer F. 1971; On the role of quinones in bacterial electron transport. Differential roles of ubiquinone and menaquinone in Proteus rettgeri. European Journal of Biochemistry 21:322–333
     [Google Scholar]
  7. Reeves H. C., Rabin R., Wegener W. S., Ajl S. J. 1971; Assay of enzymes of the tricarboxylic acid and glyoxalate cycles. Methods in Microbiology 6A:425–462
     [Google Scholar]
  8. Roberts R. B., Cowie D. B., Anderson P. H., Bolton E. T., Britten R. J. 1955 Studies on Biosynthesis in Escherichia coli. Carnegie Institution Publication 607. Washington DC: Carnegie Institution;
     [Google Scholar]
  9. Scrutton M. C. 1971; Assay of enzymes of CO2 metabolism. Methods in Microbiology 6A:479–541
     [Google Scholar]
  10. Sims W. 1970; Oral haemophili. Journal of Medical Microbiology 3:615–625
     [Google Scholar]
  11. Sims W. 1974; The clinical bacteriology of purulent oral infections. British Journal of Oral Surgery 12:1–12
     [Google Scholar]
  12. Tuyau J. E., Sims W. 1983; Aspects of the pathogenicity of some oral and other haemophili. Journal of Medical Microbiology 16:467–475
     [Google Scholar]
  13. Warringa M.G.P.J., Smith O. H., Giuditta A., Singer T. P. 1958; Studies on succinic dehydrogenase VIII. Isolation of a succinic dehydrogenase-fumaric reductase from an obligate anaerobe. Journal of Biological Chemistry 230:97–109
     [Google Scholar]
  14. White D. C. 1966; The obligatory involvement of the electron transport system in the catabolic metabolism of Haemophilus parainfluenzae. Antonie van Leeuwenhoek 32:139–158
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-7-1787
Loading
The Acid End-products of Glucose Metabolism of Oral and Other Haemophili
Microbiology 130, 1787 (1984); https://doi.org/10.1099/00221287-130-7-1787
/content/journal/micro/10.1099/00221287-130-7-1787
/content/journal/micro/10.1099/00221287-130-7-1787
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error