1887

Abstract

Antigenic substances from livers of mice infected with Tyzzer's disease were purified by means of sucrose density gradient zonal centrifugation and affinity column chromatography using antiserum and checking antigenicity with the complement fixation test. Fractions obtained from zonal centrifugation fell into three main groups with different molecular weights, two of which (Fr. I and Fr. 11) positively reacted with antiserum in the complement fixation test. Both fractions were further purified by affinity column chromatography. The molecular weights of the main antigenic substances derived from Fr. I and Fr. I1 were determined to be about 52000 and 66000, respectively, by means of SDS-PAGE.

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/content/journal/micro/10.1099/00221287-130-7-1757
1984-07-01
2021-05-12
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References

  1. Allen A. M., Canaway J. R., Moore T. D., Kinard R. F. 1965; Tyzzer’s disease syndrome in laboratory rabbits. American Journal of Pathology 46:859–881
    [Google Scholar]
  2. Casey H. L.Other Members of the Laboratory Branch, Communicable Disease Center, US Public Health Service 1969; Laboratory branch complement fixation method. In Standardized Diagnostic Complement Fixation Method and Adaptation to Micro Test pp. 1–34 Public Health Monograph no. 74, USA
    [Google Scholar]
  3. Craigie J. 1966; Bacillus piliformis (Tyzzer) and Tyzzer’s disease of the laboratory mouse. I. Propagation of the organism in embryonated eggs. Proceedings of the Royal Society B165:35–60
    [Google Scholar]
  4. Cuatrecasas P., Wllehek M., Anfensen C. B. 1968; Selective enzyme purification by affinity chromatography. Proceedings of the National Academy of Sciences of the United States of America 61:636–643
    [Google Scholar]
  5. Fujiwara K. 1967; Complement fixation reaction and agar gel double diffusion test in Tyzzer’s disease of mice. Japanese Journal of Microbiology 11:103–117
    [Google Scholar]
  6. Fujiwara K. 1978; Tyzzer’s disease. Japanese Journal of Experimental Medicine 48:467–480
    [Google Scholar]
  7. Fujiwara K., Yamada A., Ogawa H. 1971; Comparative studies on the Tyzzer’s organism from rats and mice. Japanese Journal of Experimental Medicine 41:125–133
    [Google Scholar]
  8. Fujiwara K., Kurashina H., Magaribuchi T., Takenaka S., Yokoiyama S. 1973; Further observation on the difference between Tyzzer’s organism from mice and those from rats. Japanese Journal of Experimental Medicine 43:307–315
    [Google Scholar]
  9. Fujiwara K., Takasaki Y., Kubokawa K., TakenAKA S., Kubo M., Sato K. 1974; Pathogenic and antigenic properties of the Tyzzer’s organisms from feline and hamster cases. Japanese Journal of Experimental Medicine 44:365–372
    [Google Scholar]
  10. Harrington D. D. 1976; Bacillus piliformis infection (Tyzzer’s disease) in two foals. Journal of the American Veterinary Medical Association 168:58–60
    [Google Scholar]
  11. Hashimoto F., Horigome T., Kanbayashi M., Yoshida K., Sugano H. 1983; An improved method for separation of low-molecular-weight polypeptides by electrophoresis in sodium dodecyl sulfate-polyacrylamide gel. Analytical Biochemistry 129:192–199
    [Google Scholar]
  12. Hunter B. 1971; Eradication of Tyzzer’s disease in colony of barrier-maintained mice. Iziboratory Animals 5:271–276
    [Google Scholar]
  13. Kawamura A.JR. 1977; Preparation of labelled antibody and staining method. In Fluorescent Antibody Techniques and Their Applications, 2nd edn. pp. 37–6677–99 Edited by Kawamura A. Tokyo: University of Tokyo Press;
    [Google Scholar]
  14. Kawamura S., Taguchi F., Ishida T., Nakayama M., Fujiwara K. 1983; Growth of Tyzzer’s organism in primary monolayer cultures of adult mouse hepatocytes. Journal of General Microbiology 129:277–283
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, luondon 277:680–685
    [Google Scholar]
  16. Mayer M. M. 1961; Complement and complement fixation. In Experimental Immunochemistry, 2nd edn. p. 149 Edited by Kabat E. A., Mayer M. M. . Springfield, Illinois: Charles C. Thomas;
    [Google Scholar]
  17. Merril C. R., Goldman D., Sedman S. A., Ebert M. H. 1981; Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 211:1437–1438
    [Google Scholar]
  18. Niven J. S. F. 1968; Tyzzer’s disease in laboratory animals. Zeitschrift für Versuchstierkunde 10:168–174
    [Google Scholar]
  19. Right F. L., Jackson E. B., Smadel J. E. 1947; Observations of Tyzzer’s disease in mice. American Journal of Pathology 23:627–635
    [Google Scholar]
  20. Stedman M. A., Buggi T. J. 1970; Spontaneous Tyzzer’s disease in a rat. Laboratory Animal Care 20:743–746
    [Google Scholar]
  21. Tyzzer E. E. 1917; A fatal disease of the Japanese waltzing mouse caused by a spore-bearing bacillus {Bacillus piliformis, n.sp.). Journal of Medical Research 37:307–308
    [Google Scholar]
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