1887

Abstract

The aminoglycoside phosphotransferase (APH)(3′)(5″)-III has been characterized from BM4200, which is resistant to high levels of aminoglycosides. The phosphotransferase was apparently chromosomally-encoded and was responsible for the high-level resistance. The enzyme was not notably pH-dependent, was heterogeneous after isoelectric focusing, with pI values of approximately 4·8 and 5·1, and had an apparent molecular weight of 32500 after SDS-PAGE.

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1984-07-01
2021-10-24
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References

  1. Barnes W. M. 1976; Plasmid detection and sizing in single colony lysates. Science 195:
    [Google Scholar]
  2. Buu-Hoï A., Horodniceanu T. 1980; Conjuga-tive transfer of multiple antibiotic resistance mark-ers in Streptococcus pneumoniae. Journal of Bacteriology 143:313–320
    [Google Scholar]
  3. Carlier C., Courvalin P. 1982; Resistance of streptococci to aminoglycoside-aminocyclitol antibiotics. In Microbiology-1982 pp. 162–166 Schlessinger D. Edited by Washington, D.C.: American Society for Microbiology.;
    [Google Scholar]
  4. Collatz E., Carlier C., Courvalin P. 1983; The chromosomal 3′,5″-aminoglycoside phosphotransferase in Streptococcus pneumoniae is closely related to its plasmid-coded homologs in Streptococcus faecalis and Staphylococcus aureus. Journal of Bacteriology 156:1373–1377
    [Google Scholar]
  5. Courvalin P., Davies J. 1977; Plasmid-mediated aminoglycoside phosphotransferase of broad substrate range that phosphorylates amikacin. Antimicrobial Agents and Chemotherapy 11:619–624
    [Google Scholar]
  6. Courvalin P., Fiandt M. 1980; Aminoglycosidemodifying enzymes of Staphylococcus aureus: expression in Escherichia coli. . Gene 9:247–269
    [Google Scholar]
  7. Courvalin P., Fiandt M., Davies J. 1978; DNA relationships between genes coding for aminoglycoside-modifying enzymes from antibiotic- producing bacteria and R plasmids. In Microbiology- 1978 pp. 262–266 Schlessinger D. Edited by Washington, D.C.: American Society for Microbiology.;
    [Google Scholar]
  8. Courvalin P., Carlier C., Collatz E. 1979; Structural and functional relationships between plasmid-mediated aminocyclitol-modifying enzymes from Gram-positive and Gram-negative bacteria. Program abstract, 11th Lunteren Lecture, Lunteren, The Netherlands,. abstract no. 85:
    [Google Scholar]
  9. Courvalin P., Carlier C., Collatz E. 1980; Plasmid-mediated resistance to aminocyclitol antibiotics in group D streptococci. Journal of Bacteriology 143:541–551
    [Google Scholar]
  10. Eckhardt T. 1978; A rapid method for the identification of plasmid deoxyribonucleic acid in bacteria. Plasmid 1:584–588
    [Google Scholar]
  11. Franke A. E., Clewell D. B. 1981; Evidence for a chromosome-borne resistance transposon (Tn9/6) in Streptococcus faecalis that is capable of ′conjugal’ transfer in the absence of a conjugative plasmid. Journal of Bacteriology 145:494–502
    [Google Scholar]
  12. Goldstein F. W., Boisivon A., Leclerc P., Acar J. F. 1977; Sensibilite d’hémophilus sp. aux anti-biotiques. Transfert de résistance à Escherichia coli. Pathologie biologie 25:323–332
    [Google Scholar]
  13. Guild W. R., Hazum S., Smith M. D. 1981; Chromosomal location of conjugative R determinants in a multiply resistant strain of Streptococcus pneumoniae. . Plasmid 5:226
    [Google Scholar]
  14. Haas M. J., Dowding J. E. 1975; Aminoglycoside-modifying enzymes. Methods in Enzymology 43:611–628
    [Google Scholar]
  15. Hershfield V., Boyer H. W., Yanofsky C., Lovett H. A., Helinsky D. R. 1974; Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA. Proceedings of the National Academy of Sciences of the United States of America 71:3455–3459
    [Google Scholar]
  16. Horodniceanu T., Bougueleret L., Bieth G. 1981; Conjugative transfer of multiple antibiotic resistance markers in beta-hemolytic group A, B, F and G streptococci in the absence of extrachromosomal deoxyribonucleic acid. Plasmid 5:127–137
    [Google Scholar]
  17. Jacob A. E., Hobbs S. J. 1974; Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var.zymogenes. Journal of Bacteriology 117:360–372
    [Google Scholar]
  18. Jacobs M. R., Koornhof H. J., Robins-Browne R. M., Stevenson C. M., Vermaak Z. A., Freiman I., Miller G., Witcomb M. A., Isaäcson M., Ward J. I., Austrian R. 1978; Emergence of multiply resistant pneumococci. New England Journal of Medicine 299:735–740
    [Google Scholar]
  19. Labigne-Roussel A., Gerbaud G. R., Courvalin P. 1981; Translocation of sequences encoding antibiotic resistance from the chromosome to a receptor plasmid in Salmonella ordonez. Molecular and General Genetics 182:390–408
    [Google Scholar]
  20. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  21. Macrina F. L., Mays T. D., Smith C. J., Welch R. A. 1981; Non-plasmid associated transfer of antibiotic resistance in Bacteroides. Journal of Antimicrobial Chemotherapy 8: suppl. D 77–86
    [Google Scholar]
  22. Malamy M. H., Tally F. P. 1981; Mechanisms of drug-resistance transfer in Bacteroides fragilis. Journal of Antimicrobial Chemotherapy 8: suppl. D 59–75
    [Google Scholar]
  23. Nelson C. A. 1971; The binding of detergents to proteins. I. The maximum amount of dodecyl sulfate bound to proteins and the resistance to binding of several proteins. Journal of Biological Chemistry 246:3895–3901
    [Google Scholar]
  24. O’farrell P. H. 1975; High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
    [Google Scholar]
  25. Shoemaker N. B., Smith M. D., Guild W. R. 1979; Organization and transfer of heterologous chloramphenicol and tetracycline resistance genes in pneumococcus. Journal of Bacteriology 139:432–441
    [Google Scholar]
  26. Smith C. J., Markowitz S. M., Macrina F. L. 1981; Transferable tetracycline resistance in Clostridium difficile. Antimicrobial Agents and Chemotherapy 19:997–1003
    [Google Scholar]
  27. Smith M. D., Guild W. R. 1979; A plasmid in Streptococcus pneumoniae. Journal of Bacteriology 137:735–739
    [Google Scholar]
  28. Smith M. D., Guild W. R. 1982; Evidence for transposition of the conjugative R-determinants of Streptococcus agalactiae B109. In Microbiology-1982 pp. 109–111 Schlessinger D. Edited by Washington, D.C.: American Society for Microbiology.;
    [Google Scholar]
  29. Steers E., Foltz E. L., Graves B. S. 1959; An inocula replicating apparatus for routine testing of bacterial susceptibility to antibiotics. Antibiotics and Chemotherapy 9:307–311
    [Google Scholar]
  30. Stiffler P. M., Sweeny H. M., Schneider M., Cohen S. 1974; Isolation and characterization of a kanamycin resistance plasmid from Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 6:516–520
    [Google Scholar]
  31. Trieu-Cuot P., Courvalin P. 1983; Nucleotide sequence of the Streptococcus faecalis plasmid gene encoding the 3′,5″-aminoglycoside phosphotransferase type III. Gene 23:331–341
    [Google Scholar]
  32. Zigelboim S., Tomasz A. 1980; Penicillin-binding proteins of multiply antibiotic-resistant South African strains of Streptococcus pneumoniae. Antimicrobial Agents and Chemotherapy 17:434–444
    [Google Scholar]
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