An Investigation into the Recycling of Gas Vesicle Protein Derived from Collapsed Gas Vesicles Free

Abstract

Gas vesicle protein may account for more than 6% of the cell dry weight and over 10% of the total cell protein in the planktonic cyanobacterium Anabaena Jlos-aquae. The gas vesicles collapsed by rising cell turgor pressure during buoyancy regulation can therefore represent a significant protein pool within this organism. We have looked for evidence of gas vesicle protein recycling. Gas vesicles were isolated from cells of A.80s-aquae that had been pressurized and then cultured in a radioactively labelled medium. The new gas vesicles that formed were less highly labelled than the new vesicles formedin cells from a second culture that had not been pressurized. The results suggest that the collapsed gas vesicles are disaggregated and that the constituent gas vesicle protein is reassembled into new gas vesicles. Proof of this will require experiments using in vitro assembly systems.

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1984-06-01
2024-03-29
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References

  1. Allison E. M., Walsby A. E. 1981; The role of potassium in the control of turgor pressure in a gas vacuolate blue-green alga. Journal of Experimental Botany 32:241–249
    [Google Scholar]
  2. Armstrong R. E., Hayes P. K., Walsby A. E. 1983; Gas vacuole formation in hormogonia of Nostocmuscorum. Journal of General Microbiology 128:263–270
    [Google Scholar]
  3. Booker M. J., Walsby A. E. 1979; The relative form resistance of straight and helical blue-green algal filaments. British Phycological Journal 14:141–150
    [Google Scholar]
  4. Buckland B., Walsby A. E. 1971; A study of the strength and stability of gas vesicles isolated from a blue-green alga. Archiv für Mikrobiologie 79:327–337
    [Google Scholar]
  5. Dinsdale M. T., Walsby A. E. 1972; The interrelations of cell turgor pressure, gas-vacuolation, and buoyancy in a blue-green alga. Journal of Experimental Botany 23:561–570
    [Google Scholar]
  6. Golterman H. L., Clymo R. S., Ohnstad M. A. M. 1978 Methods for Physical and Chemical Analysis of Fresh Waters, 2nd edn.. IBP Handbook No. 8. Oxford: Blackwell Scientific Publications
    [Google Scholar]
  7. Grant N. G., Walsby A. E. 1977; The contribution of photosynthate to turgor pressure rise in the planktonic blue-green alga Anabaena flosaquae. Journal of Experimental Botany 28:409–415
    [Google Scholar]
  8. Hill T. L., Kirschner M. W. 1982; Bioenergetics and kinetics of microtubule and actin filament assembly-disassembly. International Review of Cytology 78:1–125
    [Google Scholar]
  9. Lehmann H., Jost M. 1971; Kinetics of the assembly of gas vacuoles in the blue-green alga MicrocystisaeruginosaKuetz.emend. Elenkin. Archiv für Mikrobiologie 79:59–68
    [Google Scholar]
  10. Lehmann H., Jost M. 1972; Assembly of gas vacuoles in a cell-free system of the blue-green alga MicrocystisaeruginosaKuetz. emend. Elenkin. Archiv für Mikrobiologie 81:100–102
    [Google Scholar]
  11. Oliver R. L., Walsby A. E. 1984; Direct evidence for the role of light mediated gas vesicle collapse in the buoyancy regulation of Anabaena flos-aquae(cyanobacteria). Limnology and Oceanography (in the Press)
    [Google Scholar]
  12. Pearse B. M. F. 1980; Coated vesicles. Trends in Biochemical Sciences 5:131–134
    [Google Scholar]
  13. Pine M. J. 1972; Turnover of intracellular proteins. Annual Review of Microbiology 26:103–126
    [Google Scholar]
  14. Snyder J. A., Mcintosh J. R. 1976; Biochemistry and physiology of microtubules. Annual Review of Biochemistry 45:699–720
    [Google Scholar]
  15. Van Liere L., Walsby A. E. 1982; Interactions of cyanobacteria with light. In The Biology of the Cyanobacteria pp. 9–45 Edited by Carr N. G., Whitton B. A. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  16. Waaland J. R., Branton D. 1969; Gas vacuole development in a blue-green alga. Science 163:1339–1341
    [Google Scholar]
  17. Walker J. E., Walsby A. E. 1983; Molecular weight of gas vesicle protein from the planktonic cyanobacterium Anabaena flos-aquae and implications for structure of the vesicle. Biochemical Journal 209:809–815
    [Google Scholar]
  18. Walsby A. E. 1969; The permeability of blue-green algal gas-vacuole membranes to gas. Proceedings of the Royal Society B173:235–255
    [Google Scholar]
  19. Walsby A. E. 1971; The pressure relationships of gas vacuoles. Proceedings of the Royal Society B178:301–326
    [Google Scholar]
  20. Walsby A. E. 1977; Absence of gas vesicle protein in a mutant of Anabaena flos-aquae. Archiv für Mikrobiologie 114:167–170
    [Google Scholar]
  21. Walsby A. E. 1982; The elastic compressibility of gas vesicles. Proceedings of the Royal Society B216:353–368
    [Google Scholar]
  22. Walsby A. E., Armstrong R. E. 1979; Average thickness of the gas vesicle wall in Anabaena flosaquae. Journal of Molecular Biology 129:279–285
    [Google Scholar]
  23. Walsby A. E., Buckland B. 1969; Isolation and purification of intact gas vesicles from a blue-green alga. Nature, London 224:716–717
    [Google Scholar]
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