From a λgiWES library of the chromosome of . phages carrying DNA from the and regions were isolated. They were identified by their ability to form complementing plaques on . or mutants of K12 under selective conditions and in the presence of a helper phage. The phages complemented or mutations and could transform seven mutations in the region of the chromosome; each carried a single RI insert of about 8-2 kb. Phages complementing or mutations and carrying the equivalent genes and transformed a range of mutations in the region. The distribution of genetic markers carried by the phages suggests that the entire - cluster from through is covered in the collection of phages obtained and is carried in three RI restriction fragments of approximately 6.7, 4.7 and 2-85 kb.


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