1887

Abstract

From a gtWES library of the chromosome of . phages carrying DNA from the and regions were isolated. They were identified by their ability to form complementing plaques on . or mutants of K12 under selective conditions and in the presence of a helper phage. The phages complemented or mutations and could transform seven mutations in the region of the chromosome; each carried a single RI insert of about 8·2 kb. Phages complementing or mutations and carrying the equivalent genes and transformed a range of mutations in the region. The distribution of genetic markers carried by the phages suggests that the entire - cluster from through is covered in the collection of phages obtained and is carried in three RI restriction fragments of approximately 6·7, 4·7 and 2·85 kb.

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1984-06-01
2021-08-02
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References

  1. Anagnostopoulos C., Spizizen J. 1961; Requirements for transformation in Bacillus subtilis. Journal of Bacteriology 81:741–746
    [Google Scholar]
  2. Borenstein S., Ephrati-Elizur E. 1969; Spontaneous release of DNA in sequential genetic order by Bacillus subtilis. Journal of Molecular Biology 45:137–152
    [Google Scholar]
  3. Brammar W. J., Muir S., Mcmorris A. 1980; Molecular cloning of the gene for β-lactamase of Bacillus licheniformis and its expression in Escherichia coli. Molecular and General Genetics 178:217–224
    [Google Scholar]
  4. Chi N.-Y.W., Ehrlich S. D., Lederberg J. 1978; Functional expression of two Bacillus subtilis chromosomal genes in Escherichia coli. Journal of Bacteriology 133:816–821
    [Google Scholar]
  5. Davis R. W., Botstein D., Roth J. R. 1980; Extraction of DNA from phage β. In Advanced Bacterial Genetics: a Manual for Genetic Engineering pp. 106–107 New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  6. Dring G. J., Gould G. W. 1971; Movement of potassium during l-alanine-initiated germination of Bacillus subtilis spores. In Spore Research 1971 pp. 133–141 Barker A. N., Gould G. W., Wolf J. Edited by London: Academic Press.;
    [Google Scholar]
  7. Ferrari E., Henner D., Hoch J. A. 1981; Isolation of Bacillus subtilis genes from a Charon 4A library. Journal of Bacteriology 146:430–432
    [Google Scholar]
  8. Franklin N. C. 1971; The N operon of lambda; extent and regulation as observed in fusions to the tryptophan operon of E. coli. . In The Bacteriophage Lambda pp. 621–638 Hershey A. D. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  9. Goldschmidt E. D., Cater M. S., Matney T. S., Butler M. A., Greene A. 1970; Genetic analysis of the histidine operon in Escherichia coli K12. Genetics 66:219–229
    [Google Scholar]
  10. Henner D. J., Hoch J. A. 1980; The Bacillus subtilis chromosome. Microbiological Reviews 44:57–82
    [Google Scholar]
  11. Kawamura F., Saito H., Ikeda Y. 1979; A method for construction of specialised transducing phage pll of Bacillus subtilis. Gene 5:87–91
    [Google Scholar]
  12. Kawamura F., Mizukami T., Anzai H. 1981; Frequent deletion of Bacillus subtilis chromosomal fragment in artificially constructed p11phisA +. FEBS Letters 136:244–246
    [Google Scholar]
  13. Kreft J., Hughes C. 1982; Cloning vectors derived from plasmids and phage of Bacillus. In Current Topics in Microbiology and Immunology. 96. Gene Cloning in Organisms other than E. coli pp. 1–17 Hofschneider P. H., Goebel W. Edited by Berlin: Springer-Verlag.;
    [Google Scholar]
  14. Lafferty E., Moir A. 1977; Further studies on conditional germination mutants of Bacillus subtilis 168. In Spore Research 1976 pp. 87–105 Barker A. N., Wolf J., Ellar D. J., Dring G. J., Gould G. W. Edited by London: Academic Press.;
    [Google Scholar]
  15. Leder P., Tiemeier D., Enquist L. 1977; EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the λgtWES system. science 196:175–177
    [Google Scholar]
  16. Loenen W. A. M, Brammar W. J. 1980; A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes. Gene 20:249–259
    [Google Scholar]
  17. Mackey C. J., Zahler S. A. 1982; Insertion of bacteriophage SPβ into the citF gene of Bacillus subtilis and specialised transduction of the ilvBC-leu genes. Journal of Bacteriology 151:1222–1229
    [Google Scholar]
  18. Mahler I., Halvorson H. 1977; Transformation of Escherichia coli and Bacillus subtilis with a hybrid plasmid molecule. Journal of Bacteriology 131:374–377
    [Google Scholar]
  19. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  20. Miller J. H. 1972 Experiments in Molecular Genetics. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Mizukami T., Kawamura F., Saito H. 1980; Genetic instability of artificially constructed phage p11 carrying histidine A gene of Bacillus subtilis. Journal of General and Applied Microbiology 26:307–310
    [Google Scholar]
  22. Moir A. 1981; Germination properties of a spore- coat-defective mutant of Bacillus subtilis. Journal of Bacteriology 146:1106–1116
    [Google Scholar]
  23. Moir A. 1983; The isolation of λ transducing phages carrying the citG and gerA genes of Bacillus subtilis. Journal of General Microbiology 129:303–310
    [Google Scholar]
  24. Moir A., Brammar W. J. 1976; The use of specialised transducing phage in the amplification of enzyme production. Molecular and General Genetics 149:87–99
    [Google Scholar]
  25. Moir A., Smith D. A. 1983; The genetics of spore germination in Bacillus subtilis. In Fundamental and Applied Aspects of Spores pp. 89–100 Dring G. J., Ellar D. J., Gould G. W. Edited by London: Academic Press.;
    [Google Scholar]
  26. Moir A., Lafferty E., Smith D. A. 1979; Genetic analysis of spore germination mutants of Bacillus subtilis 168: the correlation of phenotype with map location. Journal of General Microbiology 111:165–180
    [Google Scholar]
  27. Murray N. E., Mandura De Ritis P. 1973; DNA targets for the Escherichia coli K restriction system analysed genetically in recombinants between phages phi80 and lambda. Molecular and General Genetics 120:261–281
    [Google Scholar]
  28. Murray N. E., Brammar W. J., Murray K. 1977; Lambdoid phages that simplify the recovery of in vitro recombinants. Molecular and General Genetics 150:53–61
    [Google Scholar]
  29. Nagahari K., Sakaguchi K. 1978; Cloning of Bacillus subtilis leucine A, B and C genes with Escherichia coli plasmids and expression of the leuC gene in E. coli. Molecular and General Genetics 158:263–270
    [Google Scholar]
  30. Nester E. W., Montoya A. L. 1976; An enzyme common to histidine and aromatic acid biosynthesis in Bacillus subtilis. Journal of Bacteriology 126:699–705
    [Google Scholar]
  31. Parkinson J. S. 1968; Genetics of the left arm of the chromosome of bacteriophage lambda. Genetics 59:311–325
    [Google Scholar]
  32. Piggot P. J., Moir A., Smith D. A. 1981; Advances in the genetics of Bacillus subtilis differentiation. In Sporulation and Germination pp. 29–39 Levinson H. S., Sonenshein A. L., Tipper D. J. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  33. Rutberg B., Hoch J. A. 1970; Citric acid cycle: gene-enzyme relationships in Bacillus subtilis. Journal of Bacteriology 104:826–833
    [Google Scholar]
  34. Sammons R. L., Anagnostopoulos C. 1982; Identification of a cloned DNA segment at a junction of chromosome regions involved in rearrangements in the trpE26 strains of Bacillus subtilis. FEMS Microbiology Letters 15:265–268
    [Google Scholar]
  35. Struhl K., Cameron J. R., Davis R. W. 1976; Functional genetic expression of eukaryotic DNA in Escherichia coli. Proceedings of the National Academy of sciences of the United States of America 73:1471–1475
    [Google Scholar]
  36. Szybalski E. H., Szybalski W. 1979; A comprehensive molecular map of bacteriophage lambda. Gene 7:217–270
    [Google Scholar]
  37. Tanaka T., Sakaguchi K. 1978; Construction of a recombinant plasmid composed of Bacillus subtilis leucine genes and a B. subtilis (natto) plasmid: its use as cloning vehicle in B. subtilis 168. Molecular and General Genetics 165:269–276
    [Google Scholar]
  38. Trowsdale J., Smith D. A. 1975; Isolation, characterisation and mapping of Bacillus subtilis 168 germination mutants. Journal of Bacteriology 123:83–95
    [Google Scholar]
  39. Warburg R. J. 1981 Some pleiotropic mutations affecting spore germination in Bacillus subtilis 168. PhD thesis University of Birmingham, UK.:
    [Google Scholar]
  40. Warburg R. J.Jr Moir A. 1981; Properties of a mutant of Bacillus subtilis 168 in which spore germination is blocked at a late stage. Journal of General Microbiology 124:243–253
    [Google Scholar]
  41. Ward J. B.Jr Zahler S. A. 1973a; Genetic studies of leucine biosynthesis in Bacillus subtilis. Journal of Bacteriology 116:719–726
    [Google Scholar]
  42. Ward J. B.Jr Zahler S. A. 1973b; Regulation of leucine biosynthesis in Bacillus subtilis. Journal of Bacteriology 116:727–735
    [Google Scholar]
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