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The S1 homothallic wild-type strain of Nectria haematococca produces grey-purplish colonies on PDA medium, due to synthesis of fusarubin and related naphthazarins. Seventeen yellow, beige or white mutants were isolated. On the basis of recombination tests they were divided into six distinct chromosomal regions. Four of them, designated befB. befC. belB and belC. may be regarded as regulatory genes which uncouple the processes of ageing and pigmentation that are normally associated in the wild-type. The two others, alyA and yelJ-beiW. control the biosynthesis of naphthazarins. Mutations in alyA were found epistatic to yelJ and beiW mutations, indicating that the alyA locus acts on an earlier biosynthetic step than the yelJ -beiW region. The latter was shown to comprise two closely linked genes, yielding yellow or beige colours when mutated. Analysis of polar mutations suggests that they form a single unit of transcription. One of these two genes probably controls a late oxidation step in naphthazarin biosynthesis.
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