1887

Abstract

An endodeoxyribonuclease specific to apurinic sites in DNA was purified 12000-fold from vegetative cells of the anaerobic thermophilic bacterium, strain IFO 13698. The enzyme specifically hydrolyses phosphodiester bonds of apurinic double-stranded DNA, without action on normal, alkylated, or single-stranded depurinated DNA. The endodeoxyribonuclease has a molecular weight of about 18500 and a sedimentation value of 2·2S. The enzyme has an optimum pH of 7·5–8·0 and an optimum temperature of 55 °C. The half-life of purified enzyme is 55 min at 60 °C but it can be heated at 60 °C for at least 60 min without loss of activity in the presence of BSA. The purified enzyme has an absolute requirement for Mg or Mn, and is inhibited by EDTA. Enzyme activity is completely inhibited by 0·5 -NaCl or 1 m--chloromercuribenzoate. All these properties differ from those of apurinic/apyrimidinic endodeoxyribonucleases isolated from . and .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-6-1525
1984-06-01
2022-01-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/6/mic-130-6-1525.html?itemId=/content/journal/micro/10.1099/00221287-130-6-1525&mimeType=html&fmt=ahah

References

  1. Bibor V., Verly W. G. 1978; Purification and properties of the endonuclease specific for apurinic sites of Bacillus stearothermophilus. Journal of Biological Chemistry 253:850–855
    [Google Scholar]
  2. Brent T. P. 1976; Purification and characterization of human endonuclease specific for damaged DNA. Biochimica et biophysica acta 454:172–183
    [Google Scholar]
  3. Brewer J. H. 1940; A clear liquid medium for the aerobic cultivation of anaerobes. Journal of Bacteriology 39:10
    [Google Scholar]
  4. Clements J. E., Rogers S. G., Weiss B. 1978; A DNase apurinic/apyrimidinic site associated with exonuclease III of Hemophilus influenzae. Journal of Biological Chemistry 253:2990–2999
    [Google Scholar]
  5. Davis B. J. 1964; Disc electrophoresis. II. Methods and application to human serum proteins. Annals of the New York Academy of sciences 121:404–427
    [Google Scholar]
  6. Drake J. W., Baltz R. H. 1976; The biochemistry of mutagenesis. Annual Review of Biochemistry 45:11–37
    [Google Scholar]
  7. Greer S., Zamenhof S. 1962; Studies on depurination of DNA by heat. Journal of Molecular Biology 4:123–141
    [Google Scholar]
  8. Hecht R., Thielman W. H. 1978; Purification and characterization of an endonuclease from Micrococcus luteus that acts on depurinated and carcinogen modified DNA. European Journal of Biochemistry 89:607–618
    [Google Scholar]
  9. Inoue T., Kada Y. 1978; Purification and properties of a Bacillus subtilis endonuclease specific for apurinic sites in DNA. Journal of Biological Chemistry 253:8559–8563
    [Google Scholar]
  10. Kadota H., Uchida A., Sako Y., Harada K. 1978; Heat-induced DNA injury in spores and vegetative cells of Bacillus subtilis. In Spores VII pp. 27–30 Chambliss G., Vary J. C. Edited by Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  11. Kane C. M., Linn S. 1981; Purification and characterization of an apurinic/apyrimidinic endonuclease from HeLa cells. Journal of Biological Chemistry 256:3405–3414
    [Google Scholar]
  12. Kuebler J. P., Goldthwait D. A. 1977; An endonuclease from calf liver specific for apurinic sites in DNA. Biochemistry 16:1370–1377
    [Google Scholar]
  13. Kunkel Y. A., Shearman C. M., Loeb L. A. 1981; Mutagenesis in vitro by depurination of ɸX174 DNA. Nature; London: 291349–351
    [Google Scholar]
  14. Lindahl T. 1979; DNA glycosylases, endonuclease for apurinic/apyrimidinic sites and base excision- repair. Progress in Nucleic Acid Research and Molecular Biology 22:135–192
    [Google Scholar]
  15. Lindahl T., Karlström O. 1973; Heat-induced depyrimidination of deoxyribonucleic acid in neutral solution. Biochemistry 12:5151–5154
    [Google Scholar]
  16. Lindahl T., Nyberg B. 1972; Rate of depurination of native deoxyribonucleic acid. Biochemistry 11:3610–3618
    [Google Scholar]
  17. Linsley W. S., Penhoet E. E., Linn S. 1977; Human endonuclease specific for apurinic/apyrimidinic sites in DNA. Journal of Biological Chemistry 252:1235–1242
    [Google Scholar]
  18. Litman R. M. 1968; A deoxyribonucleic acid polymerase from Micrococcus luteus (Micrococcus lyso- deikticus) isolated on deoxyribonucleic acid-cellulose. Journal of Biological Chemistry 243:6222–6233
    [Google Scholar]
  19. Ljungquist S. 1977; A new endonuclease from Escherichia coli acting at apurinic sites in DNA. Journal of Biological Chemistry 252:2808–2814
    [Google Scholar]
  20. Ljungquist S., Lindahl T. 1974; A mammalian endonuclease specific for apurinic sites in doublestranded deoxyribonucleic acid. Journal of Biological Chemistry 249:1530–1535
    [Google Scholar]
  21. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  22. Martin R. G., Ames B. N. 1961; A method for determining the sedimentation behavior of enzyme : application to protein mixture. Journal of Biological Chemistry 236:1372–1379
    [Google Scholar]
  23. Miyazawa Y., Thomas C. A. Jr 1965; Nucleotide composition of short segments of DNA molecules. Journal of Molecular Biology 11:223–237
    [Google Scholar]
  24. Pierre J., Laval J. 1980; Micrococcus luteus endonuclease for apurinic/apyrimidinic sites in deoxyribonucleic acid. Biochemistry 19:5018–5024
    [Google Scholar]
  25. Sako Y., Uchida A., Kadota H. 1980a; Isolation and characterization of a magnesium-dependent apurinic site specific endodeoxyribonuclease from Bacillus subtilis. Agricultural and Biological Chemistry 44:567–574
    [Google Scholar]
  26. Sako Y., Uchida A., Kadota H. 1980b; Determination of cleavage sites of APendodeoxy- ribonuclease from Bacillus subtilis. . Agricultural and Biological Chemistry 44:1713–1715
    [Google Scholar]
  27. Sako Y., Uchida A., Kadota H. 1981; Apurinic/ apyrimidinic endodeoxyribonuclease level during the life cycle of Bacillus subtilis. Agricultural and Biological Chemistry 45:337–339
    [Google Scholar]
  28. schaaper R. M., Loeb L. A. 1981; Depurination causes mutation in SOS-induced cells. Proceedings of the National Academy of sciences of the United States of America 78:1773–1777
    [Google Scholar]
  29. Shearman C. W., Loeb L. A. 1979; Effects of depurination on the fidelity of DNA synthesis. Journal of Molecular Biology 128:197–218
    [Google Scholar]
  30. Svachilova L., Satava J., Veleminsky J. 1978; A barley endonuclease specific for apurinic DNA. European Journal of Biochemistry 87:215–220
    [Google Scholar]
  31. Teebor G. W., Dukor N. J. 1975; Human endonuclease activity for DNA apurinic sites. Nature; London: 258544–547
    [Google Scholar]
  32. Thibodeau L., Bricteux S., Verly W. G. 1980; Purification and properties of the major apurinic/ apyrimidinic endodeoxyribonuclease of rat-liver chromatin. European Journal of Biochemistry 110:379–385
    [Google Scholar]
  33. Thielman H. W., Hess U. 1981; Apurinic endonuclease from Saccharomyces cerevisiae. Biochemical Journal 195:407–417
    [Google Scholar]
  34. Verly W. G. 1972; An endonuclease for depurinated DNA in Escherichia coli. . Canadian Journal of Biochemistry 50:217–224
    [Google Scholar]
  35. Verly W. G., Rassart E. 1975; Purification of Escherichia coli endonuclease specific for apurinic sites in DNA. Journal of Biological Chemistry 250:8214–8219
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-6-1525
Loading
/content/journal/micro/10.1099/00221287-130-6-1525
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error