RT Journal Article SR Electronic(1) A1 Loper, Joyce E. A1 Orser, Cindy S. A1 Panopoulos, Nickolas J. A1 Schroth, Milton N.YR 1984 T1 Genetic Analysis of Fluorescent Pigment Production in Pseudomonas syringae pv. syringae JF Microbiology, VO 130 IS 6 SP 1507 OP 1515 DO https://doi.org/10.1099/00221287-130-6-1507 PB Microbiology Society, SN 1465-2080, AB Genes involved in the biosynthesis of a fluorescent pigment by Pseudomonas syringae pv. syringae (P. s. syringae) JL2000 were investigated. A genomic library of this strain was constructed using the broad host range cosmid vector, pLAFRl. Nonfluorescent (Flu−) mutants of JL2000, defective in the biosynthesis of a fluorescent pigment, were obtained after ethyl methanesulphonate mutagenesis. Individual recombinant plasmids from the genomic library were introduced into Flu− mutants. Of a total of 146 Flu− mutants, 36 were restored to fluorescence following matings with individual recombinant colonies in the genomic library. Four separate fluorescence restoration groups, each comprised of 5 to 11 Flu− mutants restored to fluorescence by one of four structurally distinct recombinant plasmids, were identified. Whereas the 36 Flu− mutant strains differed in their abilities to grow on an iron-deficient medium, wild-type P. s. syringae strain JL2000 and all Flu+ transconjugants from these 36 crosses grew on an iron-deficient medium. These results indicate that at least four genes or gene clusters are involved in the production of a fluorescent pigment of P. s. syringae strain JL2000. These genes, with few exceptions, also control the ability of strain JL 2000 to grow under iron-limiting conditions., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-130-6-1507