1887

Abstract

An immunogold labelling technique was used to label the pili of the bacterium . The labelling was distinct and highly specific, and individual pili could be recognised beneath the gold probe. The labelling of somatic antigens could be distinguished from that of pilus antigens. Furthermore, labelling of fragments of cytoplasm released by cell lysis and trapped in the pili could be distinguished from pilus labelling. An antiserum that had been raised against strain 80200 (serotype N) labelled pili of strain 215 (serotype B). Double labelling experiments with this antiserum and the antiserum against strain 215 (serotype B) showed that both antisera label the same pili bundles. The ease of detection of the immunocytochemical reaction should enable this technique to be used as a routine screen for pilus antigens. It also possesses the potential for much wider applications for immunolabelling other antigens, such as viruses, that can be obtained in suspension.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-6-1481
1984-06-01
2021-07-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/6/mic-130-6-1481.html?itemId=/content/journal/micro/10.1099/00221287-130-6-1481&mimeType=html&fmt=ahah

References

  1. Bendayan M. 1982; Double immunocytochemical labelling applying the protein A-gold technique. Journal of Histochemistry and Cytochemistry 30:81–85
    [Google Scholar]
  2. Every D., Skerman T. M. 1982; Protection of sheep against experimental footrot by vaccination with pili purified from Bacteroides nodosus. New Zealand Veterinary Journal 30:156–158
    [Google Scholar]
  3. Every D., Skerman T. M. 1983; Surface structure of Bacteroides nodosus in relation to virulence and immunoprotection in sheep. Journal of General Microbiology 129:225–234
    [Google Scholar]
  4. Geuze H., Slot J., Van Der Ley P., Scheffer R., Griffith J. 1981; Use of colloidal gold particles in double-labelling immuno-electron microscopy of ultrathin frozen tissue sections. Journal of Cell Biology 89:653–665
    [Google Scholar]
  5. De Mey J., Moeremans M., Geuens G., Nuydens R., De Brabander M. 1981; High resolution light and electron microscopic localisation of tubulin with the IGS (immuno gold staining) method. Cell Biology International Reports 5:889–899
    [Google Scholar]
  6. Roth J., Bendayan M., Orci L. 1978; Ultrastructural localisation of antigens by the use of protein A- gold complex. Journal of Histochemistry and Cytochemistry 26:1074–1081
    [Google Scholar]
  7. Short J. A., Thorley C. M., Walker P. D. 1976; An electron microscope study of Bacteroides nodosus. Ultrastructure of organisms from primary isolates and different colony types. Journal of Applied Bacteriology 40:311–315
    [Google Scholar]
  8. Stewart D. J. 1975 Studies of morphology and antigenic structure of Fusiformis nodosus Ph.D. thesis University of Sydney; Australia:
    [Google Scholar]
  9. Stewart D. J. 1978; The role of various antigenic fractions of Bacteroides nodosus in eliciting protection against footrot in vaccinated sheep. Research in Veterinary Science 24:14–19
    [Google Scholar]
  10. Stewart D. J., Clark B. L., Peterson J. E., Griffiths D. A., Smith E. F. 1982; Importance of pilus-associated antigen in Bacteroides nodosus vaccines. Research in Veterinary Science 32:140–147
    [Google Scholar]
  11. Thorley C. M., Egerton J. R. 1981; Comparison of alum-absorbed or non-alum-absorbed oil emulsion vaccines containing either pilate or non-pilate Bacteroides nodosus cells in inducing and maintaining resistance of sheep to experimental footrot. Research in Veterinary Science 30:32–37
    [Google Scholar]
  12. Walker P. D., Short J., Thomson R. O., Roberts D. S. 1973; The fine structure of Fusiformis nodosus with special reference to the location of antigens associated with immunogeni- city. Journal of General Microbiology 77:351–361
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-6-1481
Loading
/content/journal/micro/10.1099/00221287-130-6-1481
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error