Binding Sites for Bacterial Flagella at the Surface of the Soil Amoeba Free

Abstract

(CCAP 1534/3) was found to bind avidly the common soil bacterium . This adhesion was mediated not by pili nor by the general bacterial surface but by the polar flagella. Because of the nature of the flagellar rotary motor, the cell bodies of the attached bacteria could be seen rotating clearly. While initially bacterial binding occurred uniformly over the cell membrane of . the bacteria were soon swept posteriorly to form a cap and either endocytosed or sloughed off, still agglutinated by their flagella. Such capped amoebae would not bind if challenged immediately, indicating a depletion of flagella-binding sites. The bacteria could not bind to amoebae pre-treated with concanavalin A (Con A) even after the lectin had been capped to the uroid. However, capping of flagella-binding sites did not co-cap all the Con A-binding sites on the surface of the amoeba. The flagella-binding sites were not affected by pre-treatment with Pronase (1 mg ml) or anti- surface antibody. also bound avidly by its flagella to and, furthermore, competition experiments suggested that and adhere to a common surface site on the amoeba. The presence of sites on the cell membrane of that are specific for flagellin would enhance strongly the adsorption of motile bacteria prior to endocytosis. This would represent an excellent feeding strategy for a soil-dwelling phagotroph.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-6-1449
1984-06-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/6/mic-130-6-1449.html?itemId=/content/journal/micro/10.1099/00221287-130-6-1449&mimeType=html&fmt=ahah

References

  1. Appelbaum P. C., Hugo N., Coetzee J. N. 1971; A flagellar phage for the Proteus-Providence group. Journal of General Virology 13:153–162
    [Google Scholar]
  2. Beachey E. H.Ed. 1980; Bacterial Adherence. Receptors and Recognition series B 6 London: Chapman & Hall:
    [Google Scholar]
  3. Berger E. G., Buddecke E., Kamerling J. P., Kobata A., Paulson J.-C., Vliegenhart J. F. G. 1982; Structure, biosynthesis and functions of glycoprotein glycans. Experientia 38:1129–1162
    [Google Scholar]
  4. Bowers B., Korn E. D. 1974; Localization of lipo- phosphonoglycan on both sides of the Acanthamoeba plasma membrane. Journal of Cell Biology 62:533–540
    [Google Scholar]
  5. Brown R. C., Bass H., Coombs J. P. 1975; Carbohydrate binding proteins involved in phagocytosis by Acanthamoeba. Nature, London 254:434–435
    [Google Scholar]
  6. Condeelis J. 1979; Isolation of concanavalin A caps during various stages of formation and their association with actin and myosin. Journal of Cell Biology 80:751–758
    [Google Scholar]
  7. Culbertson C. G. 1971; The pathogenicity of soil amebas. Annual Review of Microbiology 25:231–254
    [Google Scholar]
  8. Dearborn D. G., Korn E. D. 1974; Lipophosphonoglycan of the plasma membrane of Acanthamoeba castellanii. Journal of Biological Chemistry 249:3342–3346
    [Google Scholar]
  9. Dearborn D. G., Smith S., Korn E. D. 1976; Lipophosphonoglycan of the plasma membrane of Acanthamoeba castellani: inositol and phytosphingosine content and general structural features. Journal of Biological Chemistry 251:2976–2982
    [Google Scholar]
  10. Gilboa-garber N. 1972; Inhibition of broad spectrum haemagglutinin from Pseudomonas aeruginosa by d-galactose and its derivatives. FEBS Letters 20:242–244
    [Google Scholar]
  11. Icho T., Iino T. 1978; Isolation and characterization of motile Escherichia coli mutants resistant to bacteriophage x. Journal of Bacteriology 134:854–860
    [Google Scholar]
  12. King C. A., Preston T. M. 1977; Studies of anionic sites on the cell surface of the amoeba Naegleria gruberi using cationised ferritin. Journal of Cell Science 28:133–149
    [Google Scholar]
  13. Korn E. D. 1963; Fatty acids of Acanthamoeba sp. Journal of Biological Chemistry 238:3584–3587
    [Google Scholar]
  14. Korn E. D., Wright P. L. 1973; Macromolecular composition of an ameba plasma membrane. Journal of Biological Chemistry 248:439–447
    [Google Scholar]
  15. Ofek I., Mirelman D., Sharon N. 1977; Adherence of Escherichia coli to human mucosal cells mediated by mannose receptors. Nature, London 265:623–625
    [Google Scholar]
  16. Preston T. M., King C. A. 1978; An experimental study of the interaction between the soil amoeba Naegleria gruberi and a glass substrate during amoeboid locomotion. Journal of Cell Science 34:145–158
    [Google Scholar]
  17. Ray D. L. 1951; Agglutination of bacteria: a feeding mechanism in the soil amoeba Hartmanella sp. Journal of Experimental Zoology 118:443–464
    [Google Scholar]
  18. Silverman M., Simon M. 1974; Flagellar rotation and the mechanism of bacterial motility. Nature, London 249:73–74
    [Google Scholar]
  19. Smith D. G. 1982; Bacterial appendages. In Electron Microscopy of Proteins 2 pp. 105–151 Edited by Harris J. R. London: Academic Press;
    [Google Scholar]
  20. Stevens A. R., Kilpatrick T., Willaert E., Capron A. 1977; Serologic analyses of cell-surface antigens of Acanthamoeba spp. with plasma membrane antisera. Journal of Protozoology 24:316–324
    [Google Scholar]
  21. Taylor R. B., Duffus W. P. M, Raff M. C., De Petris S. 1971; Redistribution and pinocytosis of lymphocyte surface immunoglobulin molecules induced by anti-immunoglobulin antibody. Nature New Biology 233:225–229
    [Google Scholar]
  22. Thompson J. E., Pauls K. P. 1980; Membranes of small amoebae. In Biochemistry and Physiology of Protozoa 3 pp. 207–253 Edited by Levandowsky M., Hutner S. M. New York: Academic Press;
    [Google Scholar]
  23. Visvesvara G., Jones D., Robinson N. 1975; Isolation, identification and biological characterization of Acanthamoeba polyphaga from a human eye. American Journal of Tropical Medicine and Hygiene 24:784–790
    [Google Scholar]
  24. Weisman R. A., Korn E. D. 1967; Phagocytosis of latex beads by Acanthamoeba. I. Biochemical properties. Biochemistry 6:485–497
    [Google Scholar]
  25. Wright S. J. L, Redhead K., Maudsley H. 1981; Acanthamoeba castellanii, a predator of cyanobacteria. Journal of General Microbiology 125:293–300
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-6-1449
Loading
/content/journal/micro/10.1099/00221287-130-6-1449
Loading

Data & Media loading...

Most cited Most Cited RSS feed