Two different replication determinants were found on an antibiotic resistance plasmid, pTB19, from a thermophilic bacillus. One replication determinant (designated RepA) was functional only in . whereas the other (designated RepB) functioned in both and . A deletion plasmid, pTB90, carrying the RepB derived from pTB19 coincidentally contained the specific 1-0 MDal RI fragment of a cryptic plasmid pBSO2 from . The presence of this 1.0 MDal RI fragment in various deletion plasmids from pTB90 increased transformation frequencies for 10 to 10 times and lowered plasmid copy numbers in the host strain to about one-tenth of those found for plasmids lacking this fragment.


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