1887

Abstract

The role of phospholipid metabolism in the functioning of the bacterial envelope was investigated in the chain-forming Lysophosphatidylethanolamine (LPE) which accumulated in this strain during growth was indentified as the product of phosphatidylethanolamine (PE) hydrolysis by a phospholipase A, i.e. 2-acylLPE. Isotopically labelled LPE transferred into intact mutant and parent cells by liposome/bacteria interaction was rapidly reacylated to PE. However, in the final PE/LPE ratio was lower than that in the parent, thus showing that the fate of LPE is modified. Crude cell extracts degraded LPE to a lesser extent in than in the parent but were unable to promote reacylation activity under our experimental conditions. In both strains, the lysophospholipase activity was neither calcium-dependent nor inhibited by the SH-group inhibitors HMB or CMPS, and hydrolysed 1-acylLPE as well as 2-acylLPE. These results indicate the existence of a deacylation-reacylation cycle in and show that this cycle is perturbed in cells, especially at the lysophospholipase step.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-6-1391
1984-06-01
2021-05-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/6/mic-130-6-1391.html?itemId=/content/journal/micro/10.1099/00221287-130-6-1391&mimeType=html&fmt=ahah

References

  1. Abe M., Okamoto N., Doi O., Nojima S. 1974; Genetic mapping of the locus for detergent-resistant phospholipase A (pldA) in E. coli K12. Journal of Bacteriology 119:543–546
    [Google Scholar]
  2. Ames B., Dubin D. T. 1960; Role of polyamines in the neutralisation of bacteriophage DNA. Journal of Biological Chemistry 235:769–775
    [Google Scholar]
  3. Bierbaum T. J., Bouma S. R., Huestis W. J. 1979; A mechanism of erythrocytes lysis by lysophosphatidylcholine. Biochimica et biophysica acta 555:102–110
    [Google Scholar]
  4. Cullis P, Dekruijff B. 1978; Polymorphic phase behaviour of lipid mixtures as detected by31P-NMR. Evidence that cholesterol may destabilize bilayer structure in membrane systems containing phosphatidylethanolamine. Biochimica et biophysica acta 551:248–259
    [Google Scholar]
  5. Cullis P. R., De Kruijff B. 1979; Lipid polymorphism and the functional roles of lipids in biological membranes. Biochimica et biophysica acta 559:399–420
    [Google Scholar]
  6. Doi O., Nojima S. 1975; Lysophospholipase of E, coli. Journal of Biological Chemistry 250:5208–5214
    [Google Scholar]
  7. Doi O., Nojima S. 1976; Nature of E. coli mutants deficient in detergent-resistant and/or detergent-sensitive phospholipase A. Journal of Biochemistry 80:1247–1248
    [Google Scholar]
  8. Doi O., Ohki M., Nojima S. 1972; Two kinds of phospholipase A and lysophospholipase in Escherichia coli. Biochimica et biophysica acta 260:244–258
    [Google Scholar]
  9. Duckworth D. H., Bevers E., Verkleij A. J., Op Den Kamp J. A. F, Van Deenen L. L. M. 1974; Action of phospholipase A2 and phospholipase C on E. coli. Archives of Biochemistry and Biophysics 165:379–387
    [Google Scholar]
  10. Gerritsen W. J., Van Zoelen E. J. J, Verkleij A. J., De Kruijff B., Van Deenen L. L. M. 1979; A 13C-NMR method for determination of the transbilayer distribution of phosphatidylcholine in large unilamellar, protein free and protein containing vesicles. Biochimica et biophysica acta 551:248–259
    [Google Scholar]
  11. Hardaway K. L., Buller C. S. 1979; Effect of EDTA on phospholipid and outer membrane function in E. coli. Journal of Bacteriology 137:62–68
    [Google Scholar]
  12. Homma H., Nishijima M., Kobayashi T., Okuyama H., Nojima S. 1981; Incorporation and metabolism of 2-acyllysophospholipid by E. coli K12. Biochimica et biophysica acta 663:1–13
    [Google Scholar]
  13. Jones N. C., Osborn M. J. 1977; Interaction of S. typhimurium with phospholipid vesicles. Incorporation of exogenous lipids into intact cells. Journal of Biological Chemistry 252:7398–7404
    [Google Scholar]
  14. Kaneshiro T., Marr A. G. 1961; cis-9,10-Methylenehexadecanoic acid from the phospholipids of E. coli. Journal of Biological Chemistry 236:2615–2619
    [Google Scholar]
  15. Luzzati V., Gulik Krzywicki T., Tardieu A. 1968; Polymorphism of lecithins. Nature, London 218:1031–1034
    [Google Scholar]
  16. Michel G. P. F. 1979; The changes in cell size and phospholipid composition during growth of a chainforming envC mutant of E. coli. FEBS Letters 100:258–260
    [Google Scholar]
  17. Michel G. P. F, Starka J. 1979; Phospholipase A activity with integrated phospholipid vesicles in intact cells of an envelope mutant of E. coli. FEBS tetters 108:261–265
    [Google Scholar]
  18. Mookerjea S. 1979; Effect of lysolecithin in solubilizing membrane-bound galactosyltransferases. Canadian Journal of Biochemistry 57:66–71
    [Google Scholar]
  19. Nishijima M., Nakaike S., Tamori Y., Nojima S. 1977; Detergent-resistant phospholipase A of E. coli K12. Purification and properties. European Journal of Biochemistry 73:115–124
    [Google Scholar]
  20. Ohki M., Doi O., Nojima S. 1972; Mutants of E. coli K12 deficient for detergent-resistant phospholipase A. Journal of Bacteriology 110:864–869
    [Google Scholar]
  21. Proulx P. R., Van Deenen L. L. M. 1966; Acylation of lysophospholipids by E. coli. Biochimica et biophysica acta 125:591–593
    [Google Scholar]
  22. Raetz C. R. H. 1978; Enzymology, genetics and regulation of membrane phospholipid synthesis in E. coli. Microbiological Reviews 42:614–659
    [Google Scholar]
  23. Rodolakis A., Casse F., Starka J. 1974; Morphological mutant of E. coli K12. Molecular and General Genetics 130:177–181
    [Google Scholar]
  24. Scandella C. J., Kornberg A. 1971; A membrane-bound phospholipase purified from E. coli. Biochemistry 10:4447–4456
    [Google Scholar]
  25. Starka J., Moravova J. 1970; Phospholipids and cellular division of Escherichia coli. Journal of General Microbiology 60:251–257
    [Google Scholar]
  26. Tamori Y., Nishijima M., Nojima S. 1979; Properties of purified detergent-resistant phospholipase A of E. coli. Inactivation and protection with detergents and phospholipids. Journal of Biochemistry 86:1129–1138
    [Google Scholar]
  27. Vos M. M., Op Den Kamp J. A. F, Bequerdite Quagliata S., Elsbach P. 1978; Acylation of monoacylglycerophosphoethanolamine in the inner and the outer membranes of the envelope of an E. coli K12 strain and its phospholipase A-deficient mutant. Biochimica et biophysica acta 508:165–173
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-6-1391
Loading
/content/journal/micro/10.1099/00221287-130-6-1391
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error