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β-Glucosidase activity in Cellulomonas fimi is strictly intracellular. Cell-free extracts contain at least two β-glucosidases. One of the enzymes is constitutive and it hydrolyses p -nitrophenyl-β-d-glucoside (PNPG) but not cellobiose. A second enzyme is induced about fourfold by growth on Avicel, and about sevenfold by growth on cellobiose; it hydrolyses both PNPG and cellobiose.
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