%0 Journal Article %A Gilkes, N. R. %A Kilburn, D. G. %A Langsford, M. L. %A Miller, R. C %A Wakarchuk, W. W. %A Warren, R. A. J. %A Whittle, D. J. %A Wong, W. K. R. %T Isolation and Characterization of Escherichia coli Clones Expressing Cellulase Genes from Cellulomonas fimi %D 1984 %J Microbiology, %V 130 %N 6 %P 1377-1384 %@ 1465-2080 %R https://doi.org/10.1099/00221287-130-6-1377 %I Microbiology Society, %X A sensitive immunoassay developed previously was used to isolate a series of Escherichia coli clones expressing cellulase genes from Cellulomonas fimi. The clones fell into three groups. Clones in the first group contained plasmids with a 6·6 kb insert of C. fimi DNA, were strongly antigenic, and contained low levels of CM-cellulase activity. Those in the second group contained plasmids with a 5·0 kb insert, were weakly antigenic, and contained high levels of CM-cellulase activity. Those in the third group contained plasmids with a 5·6 kb insert, were weakly antigenic, and contained low levels of CM-cellulase activity. Restriction analysis showed that the three groups carried different cellulase genes. All of these CM-cellulase activities were exported to the periplasm in E. coli. but with different efficiencies. These activities represented important components of the C. fimi cellulase complex. Their properties indicated that they were different from each other and that they probably had complementary actions. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-130-6-1377