1887

Abstract

A sensitive immunoassay developed previously was used to isolate a series of clones expressing cellulase genes from . The clones fell into three groups. Clones in the first group contained plasmids with a 6·6 kb insert of DNA, were strongly antigenic, and contained low levels of CM-cellulase activity. Those in the second group contained plasmids with a 5·0 kb insert, were weakly antigenic, and contained high levels of CM-cellulase activity. Those in the third group contained plasmids with a 5·6 kb insert, were weakly antigenic, and contained low levels of CM-cellulase activity. Restriction analysis showed that the three groups carried different cellulase genes. All of these CM-cellulase activities were exported to the periplasm in . but with different efficiencies. These activities represented important components of the cellulase complex. Their properties indicated that they were different from each other and that they probably had complementary actions.

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/content/journal/micro/10.1099/00221287-130-6-1377
1984-06-01
2021-10-16
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