1887

Abstract

Chitinase activities were detected in the supernatant and microsomal fractions from homogenized mycelium of surface-grown cultures of . Most properties of the two preparations were very similar, e.g. pH optima were 5.65 and 5.55, respectively, and values were 16.7 and 19.2 mg chitin ml, respectively. Neither activity had a cationic requirement, but both were inhibited by CuSome properties differed. On freezing, the supernatant activity remained constant over 4 d and then gradually decreased, whereas microsomal activity increased, suggesting that endogenous activation was occurring. Prolonged incubations at 30 °C also allowed endogenous activation of microsomal activity, which could be prevented by incubation with phenylmethanesulphonyl fluoride. This enzyme activity could also be activated by treatment of the microsomes with proteases such as trypsin. Inactivation occurred after treatment with phospholipases, suggesting that microsomal chitinase requires phospholipid-enzyme interactions for activity. Thus the microsomal activity behaved as a membrane-bound zymogen.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-6-1359
1984-06-01
2019-10-19
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-6-1359
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error