RT Journal Article SR Electronic(1) A1 Hinchliffe, EdwardYR 1984 T1 Cloning and Expression of a Bacillus subtilis> Endo-1,3-1,4-β-d-Glucanase Gene in Escherichia coli> K12 JF Microbiology, VO 130 IS 5 SP 1285 OP 1291 DO https://doi.org/10.1099/00221287-130-5-1285 PB Microbiology Society, SN 1465-2080, AB EcoRI fragments of DNA from Bacillus subtilis NCIB 8565, a high producer of an endo-1,3-1,4-β-d-glucanase, were ‘shot-gun’ cloned in the plasmid vector pBR325. A 3·5 kb insert, carrying single restriction sites for AvaI, BglII, ClaI, PvuI and PvuII, was shown to direct the synthesis of β-glucanase in Escherichia coli K12. Enzyme activity was demonstrated in extracellular fractions of E. coli harbouring the β-glucanase gene; however, the largest proportion (< 50%) of total enzyme activity was periplasmic in location. β-Glucanase activity and cellular location were independent of the orientation of the 3·5 kb fragment in pBR325., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-130-5-1285