1887

Abstract

RI fragments of DNA from NCIB 8565, a high producer of an endo-1,3-1,4---glucanase, were ‘shot-gun’ cloned in the plasmid vector pBR325. A 3·5 kb insert, carrying single restriction sites for I, II, I, I and II, was shown to direct the synthesis of -glucanase in K12. Enzyme activity was demonstrated in extracellular fractions of harbouring the -glucanase gene; however, the largest proportion (< 50%) of total enzyme activity was periplasmic in location. -Glucanase activity and cellular location were independent of the orientation of the 3·5 kb fragment in pBR325.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-5-1285
1984-05-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/5/mic-130-5-1285.html?itemId=/content/journal/micro/10.1099/00221287-130-5-1285&mimeType=html&fmt=ahah

References

  1. Bamforth C. W. 1982; Barley β-glucans. Their role in malting and brewing. Brewers DigestJune22–35
    [Google Scholar]
  2. Barras D. R., Moore A. E., Stone B. A. 1969; Enzyme substrate relationships among β-glucan hydrolases. In Celluloses and Their Applications 156th Meeting of the American Chemical Society September1112 1968 pp. 105–138 Atlantic City: American Chemical Society;
    [Google Scholar]
  3. Bertani G. 1951; Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. . Journal of Bacteriology 62:293–300
    [Google Scholar]
  4. Birnboim H. C., Doly J. 1980; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  5. Blobel G., Dobberstein B. 1975; Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. Journal of Cell Biology 67:835–851
    [Google Scholar]
  6. Bolivar F. 1978; Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique EcoRI sites for selection of EcoRI generated recombinant DNA molecules. Gene 4:121–136
    [Google Scholar]
  7. Boyer H. W., Roulland-Dussoix D. 1969; A complementation analysis of the restriction and modification of DNA in Escherichia coli. . Journal of Molecular Biology 41:459–472
    [Google Scholar]
  8. Cantwell B. A. 1983; Molecular cloning and expression of a Bacillus subtilis β-glucanase gene in Escherichia coli. . Gene 23:211–219
    [Google Scholar]
  9. Clewell D. B., Helinski D. R. 1969; Supercoiled circular DNA-protein complex in E. coli. Purification and induced conversion to an open circular DNA form. Proceedings of the National Academy of sciences of the United States of America 62:1159–1166
    [Google Scholar]
  10. Cornelis P., Dlgneffe C., Wlllemot K. 1982; Cloning and expression of a Bacillus coagulans amylase gene in Escherichia coli. . Molecular and General Genetics 186:507–511
    [Google Scholar]
  11. Godfrey T. 1983a; Brewing. In Industrial Enzymo-logy pp. 221–259 Godfrey T., Reichelt J. Edited by London: Macmillan;
    [Google Scholar]
  12. Godfrey T. 1983b; Comparison of key characteristics of industrial enzymes by type and source. In Industrial Enzymology pp. 466–502 Godfrey T., Reichelt J. Edited by London: Macmillan;
    [Google Scholar]
  13. Helling R. B., Goodman H. M., Boyer H. W. 1974; Analysis of endonuclease R. EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose gel electrophoresis. Journal of Virology 14:1235–1244
    [Google Scholar]
  14. Katz L., Kingsbury D. T., Helinski D. R. 1973; Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex. Journal of Bacteriology 114:577–591
    [Google Scholar]
  15. Kaiser K., Murray N. E. 1979; Physical characterization of the ‘Rac prophage’ in E. coli K12. Molecular and General Genetics 175:159–174
    [Google Scholar]
  16. Kreil G. 1981; Transfer of proteins across membranes. Annual Review of Biochemistry 50:317–348
    [Google Scholar]
  17. Manners D. J., Wilson G. 1976; Purification of malted barley endo-β-d-glucanase by ion exchange chromatography: some properties of an endo-barley-β-d-glucanase. Carbohydrate Research 48:255–264
    [Google Scholar]
  18. Martin H. L., Bamforth C. W. 1983; Application of a radial diffusion assay for the measurement of β-glucanase in malt. Journal of the Institute of Brewing 89:34–37
    [Google Scholar]
  19. Moscatelli E. A., Ham E. A., Rickes E. L. 1961; Enzymatic properties of a β-glucanase from Bacillus subtilis. . Journal of Biological Chemistry 236:2858–2862
    [Google Scholar]
  20. Rickes E. L., Ham E. A., Moscatelli E. A., Ott W. H. 1962; The isolation and biological properties of a β-glucanase from B. subtilis. . Archives of Biochemistry and Biophysics 69:371–375
    [Google Scholar]
  21. Soberon X., Covarrubias L., Bolivar F. 1980; Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. Gene 9:287–305
    [Google Scholar]
  22. Teather R. M., Wood P. J. 1982; Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulytic bacteria from the bovine rumen. Applied and Environmental Microbiology 43:777–780
    [Google Scholar]
  23. Wilson G. G., Murray N. E. 1979; Molecular cloning of the DNA ligase gene from bacteriophage T4. Molecular and General Genetics 156:203–214
    [Google Scholar]
  24. Yang M., Galizzi A., Henner D. 1983; Nucleotide sequence of amylase gene from Bacillus subtilis. . Nucleic Acids Research 11:237–249
    [Google Scholar]
  25. Zahn G., Tippe-schindler R., Messer W. 1977; Transdominance of dnaA mutants in Escherichia coli. . Molecular and General Genetics 153:45–49
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-5-1285
Loading
/content/journal/micro/10.1099/00221287-130-5-1285
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error