The aspartase gene () of has been isolated in two plasmids, pGS73 and pGS94, which contain segments of bacterial DNA (12.5 and 2.8 kb, respectively) inserted into the gene of the vector pBR322. The plasmids were constructed by sequential sub-cloning from a larger Co\El- hybrid plasmid. The location of the gene confirmed predictions based on a correlation between the genetic and restriction maps of the corresponding region. The aspartase activities of plasmid-containing mutants were amplified four- to sixfold relative to parental strains. The gene product was tentatively identified as a poly-peptide of 55 000, which is somewhat larger than previous estimates ( 45 000 to 48 000) for aspartase.


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