1887

Abstract

We have isolated a mutant of , strain 590, which is blocked at stage III of sporulation. The mutation which is carried by this strain is linked to by transformation and defines a previously unidentified locus, . The locus is contiguous with the locus, in which a mutation causes a block at stage V of sporulation. We also give a detailed genetic map of the region of the chromosome.

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1984-05-01
2024-04-25
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References

  1. Brehm S. P., Le Hegarat F., Hoch J. A. 1975; Deoxyribonucleic acid-binding proteins in vegeta-tive Bacillus subtilis: alterations caused by stage 0 sporulation mutations. Journal of Bacteriology 124:977–984
    [Google Scholar]
  2. Carlton B. C. 1966; Fine-structure mapping by transformation in the tryptophan region of Bacillus subtilis. Journal of Bacteriology 91:1795–1803
    [Google Scholar]
  3. Coote J. G. 1972a; Sporulation in Bacillus subtilis. Characterization of oligosporogenous mutants and comparison of their phenotypes with those of asporogenous mutants. Journal of General Microbiology 71:1–15
    [Google Scholar]
  4. Coote J. G. 1972b; Sporulation in Bacillus subtilis. Genetic analysis of oligosporogenous mutants. Journal of General Microbiology 71:17–27
    [Google Scholar]
  5. Dunn G., Torgersen D. M., Mandelstam J. 1976; Order of expression of genes affecting septum location during sporulation of Bacillus subtilis. Journal of Bacteriology 125:776–799
    [Google Scholar]
  6. Errington J., Mandelstam J. 1983; Variety of sporulation phenotypes resulting from mutations in a single regulatory locus, spoil A, in Bacillus subtilis. Journal of General Microbiology 129:2091–2101
    [Google Scholar]
  7. Esposito M. S., Esposito R. E., Arnaud M., Halvorson H. O. 1970; Conditional mutants of meiosis in yeast. Journal of Bacteriology 104:202–210
    [Google Scholar]
  8. Ferrari F. A., Lang D., Ferrari E., Hoch J. A. 1982; Molecular cloning of the spo0B sporulation locus in bacteriophage lambda. Journal of Bacteri-ology 152:809–814
    [Google Scholar]
  9. Glenn A. R., Mandelstam J. 1971; Sporulation in Bacillus subtilis 168. Comparison of alkaline phosphatase from sporulating and vegetative cells. Biochemical Journal 123:129–138
    [Google Scholar]
  10. Hirochika H., Kobayashi Y., Kawamura F., Saito H. 1981; Cloning of sporulation gene spo0B of Bacillus subtilis and its genetic and biochemical analysis. Journal of Bacteriology 146:494–505
    [Google Scholar]
  11. Hoch J. A., Mathews J. L. 1973; Chromosomal location of pleiotropic negative sporulation muta-tions in Bacillus subtilis. Genetics 73:215–228
    [Google Scholar]
  12. Hoch J. A., Shiflett M. A., Trowsdale J., Chen S. M. H. 1978; Stage 0 genes and their products. In Spores VII pp. 127–130 Chambliss G., Vary J. C. Edited by Washington, D.C.:: American Society for Microbiology.;
    [Google Scholar]
  13. Hranueli D., Piggot P. J., Mandelstam J. 1974; Statistical estimate of the total number of operons specific for Bacillus subtilis sporulation. Journal of Bacteriology 119:684–690
    [Google Scholar]
  14. Lamont I. L., Mandelstam J. 1984; Identification of a new sporulation locus. spoIIIF, in Bacillus subtilis. Journal of General Microbiology 130:1253–1261
    [Google Scholar]
  15. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  16. Piggot P. J., Coote J. G. 1976; Genetic aspects of bacterial endospore formation. Bacteriological Reviews 40:908–962
    [Google Scholar]
  17. Ward J. B. Jr Zahler S. A. 1973; Genetic studies of leucine biosynthesis in Bacillus subtilis. Journal of Bacteriology 116:719–726
    [Google Scholar]
  18. Young M. 1976; Use of temperature-sensitive mutants to study gene expression during sporulation in Bacillus subtilis. Journal of Bacteriology 126:928–936
    [Google Scholar]
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