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Glutamine is shown to be the effector compound for the regulation of both nitrogenase and glutamine synthetase activities in Rhodopseudomonas sphaeroides f. sp. denitrificans. Glutamine synthetase and nitrogenase (components I and II) in this bacterium have similar properties to those from Rhodopseudomonas capsulata. Ammonia-shock treatment of washed cells, which resulted in an accumulation of [14C]glutamine from [14C]glutamate, was accompanied by an adenylylation of glutamine synthetase and an inhibition of nitrogenase. Treatment of cells with azaserine (which inhibits glutamate synthase) enhanced the accumulation of labelled glutamine from [14C]glutamate and 15NH4C1. Glutamine inhibited the nitrogenase activity of azaserine-treated cells to a greater extent than that of untreated cells.
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