Extracellular membrane particles were isolated from the cell-free culture liquid of Acinetobacter calcoaceticus 69-V grown on n-alkanes. It was demonstrated by means of an antiserum raised against intact cells of A. calcoaceticus 69-V grown on hexadecane that the particles were probably derived from the surface membrane fraction of the bacteria. The accumulation by the bacteria of radioactivity originating from [14C]hexadecane solubilized in the particles was studied. The apparent Km value for the uptake of solubilized hexadecane was 33 µm and the Vmax value was 1·2 × 10−9 mol min−1 (mg bacterial protein)−1. The Km value for the uptake of free hexadecane was approximately 10 times higher, indicating the possible role of particles as transport vehicles for the insoluble substrate. Accumulation of radioactivity was inhibited by KCN and NaN3, suggesting an energy-dependent uptake process.
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