1887

Abstract

Novozym 234, a commercially available enzyme, has been used successfully to prepare protoplasts from yeast-like cells, mycelium and chlamydospores of the polymorphic fungus . Use of the density gradient material Percoll after wall digestion resulted in preparations of high purity that were metabolically active. Size distributions of protoplasts were determined easily using Coulter analysis.

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/content/journal/micro/10.1099/00221287-130-5-1031
1984-05-01
2020-11-23
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-5-1031
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