%0 Journal Article %A Biebricher, Christof K. %A Düker, Eva-Maria %T F and Type 1 Piliation of Escherichia coli %D 1984 %J Microbiology, %V 130 %N 4 %P 951-957 %@ 1465-2080 %R https://doi.org/10.1099/00221287-130-4-951 %I Microbiology Society, %X Summary: F and type 1 piliation of various Escherichia coli K12 Hfr and F+ strains was re-examined by using a new visualization assay. In anaerobic cultures in rich media, bacteria were well F piliated throughout all growth phases. In aerobic cultures in rich media, F piliation reached a maximum in the mid-exponential phase. The yield of pili was up to 15 mg 1−1. In aerated cultures. F pili disappeared in the late exponential phase. In rich media F pili were on average 10-20 μm long; in synthetic media they were an order of magnitude shorter, and less numerous. Addition of metabolic poisons (NaCN, NaN3, Na3 AsO4, phenylethyl alcohol) and starving the bacteria caused rapid disappearance of F pili under aerobic conditions, but had no influence on F piliation under anaerobic conditions. Type 1 piliation was not influenced by these drugs or by an alteration of growth conditions. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-130-4-951