1887

Abstract

Summary: F and type 1 piliation of various K12 Hfr and F strains was re-examined by using a new visualization assay. In anaerobic cultures in rich media, bacteria were well F piliated throughout all growth phases. In aerobic cultures in rich media, F piliation reached a maximum in the mid-exponential phase. The yield of pili was up to 15 mg 1. In aerated cultures. F pili disappeared in the late exponential phase. In rich media F pili were on average 10-20 μm long; in synthetic media they were an order of magnitude shorter, and less numerous. Addition of metabolic poisons (NaCN, NaN, Na AsO, phenylethyl alcohol) and starving the bacteria caused rapid disappearance of F pili under aerobic conditions, but had no influence on F piliation under anaerobic conditions. Type 1 piliation was not influenced by these drugs or by an alteration of growth conditions.

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1984-04-01
2021-08-02
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References

  1. Achtman M., Manning P., Edelbluthe E., Herrlich P. 1979; Export without proteolytic processing of inner and outer membrane proteins encoded by F sex factor tra cistrons in Escherichia coli minicells. Proceedings of the National Academy of Sciences of the United States of America 76:4837–4841
    [Google Scholar]
  2. Beard J. B., Connolly J. C. 1975; Detection of a protein, similar to the sex pilus subunit in the outer membrane of Escherichia coli cells, carrying a derepressed F-like R factor. Journal of Bacteriology 122:59–65
    [Google Scholar]
  3. Beard J. P., Howe T. G. B., Richmond M. H. 1972; Purification of sex pili from Escherichia coli carrying a derepressed F-like R factor. Journal of Bacteriology 111:814–820
    [Google Scholar]
  4. Biebricher C. K., Düker E.-M. 1984; Light-microscopic visualization of F and type 1 pili. Journal of General Microbiology 130:941–949
    [Google Scholar]
  5. Brinton C. C. 1965; The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in Gram negative bacteria. Transactions of the New York Academy of Sciences 27:1003–1054
    [Google Scholar]
  6. Brinton C. C. 1971; The properties of sex pili, the viral nature of “conjugal” genetic transfer systems, and some possible approaches to the control of bacterial drug resistance. Critical Reviews of Micro-biology 1:105–160
    [Google Scholar]
  7. Brinton C. C., Beer H. 1967; The interaction of male-specific bacteriophages with F-pili. In The Molecular Biology of Viruses pp. 251–289 Colter J. C., Paranchych W. Edited by New York: Academic Press;
    [Google Scholar]
  8. Curtiss R., Caro L. G., Allison D. P., Stallions D. R. 1969; Early stages of conjugation in Escherichia coli. Journal of Bacteriology 100:1091–1104
    [Google Scholar]
  9. Date T., Inuzuka M., Tomoeda M. 1977; Purification and characterization of F-pili from Escherichia coli. Biochemistry 16:5579–5585
    [Google Scholar]
  10. Drèze A. 1960; La stabilite du tryptophane au cours de l’hydrolyse alcaline effectuee en presence d’hydrates de carbone. Bulletin de la Societe de chimie biologique 42:407–417
    [Google Scholar]
  11. Dürwald H., Hoffmann-Berling H. 1968; Endonuclease I-deficient and ribonuclease I-deficient Escherichia coli mutants. Journal of Molecular Biology 34:331–346
    [Google Scholar]
  12. Eisenstein B. I., Beachey E. H., Solomon S. S. 1981; Divergent effects of cyclic adenosine-3′,5′- monophosphate on formation of type 1 fimbriae in different K-12 strains of Escherichia coli. Journal of Bacteriology 145:620–623
    [Google Scholar]
  13. Frost L. S., Paranchych W. 1977; Composition and molecular weight of pili purified from Pseudomonas aeruginosa K. Journal of Bacteriology 131:259–269
    [Google Scholar]
  14. Helmuth R., Achtman M. 1978; Cell-cell interactions in conjugating Escherichia coli: purification of F pili with biological activity. Proceedings of the National Academy of Sciences of the United States of America 15:1237–1241
    [Google Scholar]
  15. Ippen K. A., Valentine R. C. 1965; An assay for the male substance (F pili) of Escherichia coli K12. Biochemical and Biophysical Research Communications 21:21–27
    [Google Scholar]
  16. Kjeldgaard N. O., Gausing K. 1974; Regulation of biosynthesis of ribosomes. In Ribosomes pp. 369–392 Nomura M., Tissieres A., Lengyel P. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Korhonen T. K., Lounatmaa K., Ranta H., Kuusi N. 1980; Characterization of type 1 pili of Salmonella typhimurium LT2. Journal of Bacteriology 144:800–805
    [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227660–685
    [Google Scholar]
  19. Maccacaro G. A., Turri M. 1959; Studi sulle fimbrie batteriche. Osservazioni microelettroforetiche. Giornale di microbiologia 7:21–36
    [Google Scholar]
  20. Minkley E. G., Polen S., Brinton C. C. 1976; Identification of the structural gene for F-pilin. Journal of Molecular Biology 108:111–121
    [Google Scholar]
  21. Moore D., Sowa B. A., Ippen-Ihler K. 1981; Location of an F-pilin pool in the inner membrane. Journal of Bacteriology 146:251–259
    [Google Scholar]
  22. Moore S. 1963; On the determination of cystine as cysteic acid. Journal of Biological Chemistry 238:235–237
    [Google Scholar]
  23. Novotny C. P., Lavin K. 1971; Some effects of temperature on the growth of F pili. Journal of Bacteriology 107:671–682
    [Google Scholar]
  24. Novotny C. P., Taylor P., Lavin K. 1972; Effects of growth inhibitors and ultraviolet irradiation on F pili. Journal of Bacteriology 112:1083–1089
    [Google Scholar]
  25. Novotny C. P., Fives-Taylor P. 1974; Retraction of F pili. Journal of Bacteriology 117:1306–1311
    [Google Scholar]
  26. O’Callaghan R. J., Bundy L., Bradley R., Paranchych W. 1973; Unusual arsenate poisoning of the F pili of Escherichia coli. Journal of Bacteriology 115:76–81
    [Google Scholar]
  27. Stallions D. R., Curtiss R. 1972; Bacterial conjugation under anaerobic conditions. Journal of Bacteriology 111:294–295
    [Google Scholar]
  28. Swaney L. M., Liu Y. P., To Ch. M., To Ch. Ch., Ippen-Ihler K., Brinton C. C. 1977; Isolation and characterization of Escherichia coli phase variants and mutants deficient in type 1 pilus production. Journal of Bacteriology 130:495–505
    [Google Scholar]
  29. Tomoeda M., Inozuka M., Date T. 1975; Bacterial sex pili. Progress in Biophysics and Molecular Biology 30:23–56
    [Google Scholar]
  30. Wendt L., Mobach H. W. 1968; Evidence for two states of F pili. Journal of Bacteriology 97:640–646
    [Google Scholar]
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