1887

Abstract

Summary: F and type 1 piliation of various K12 Hfr and F strains was re-examined by using a new visualization assay. In anaerobic cultures in rich media, bacteria were well F piliated throughout all growth phases. In aerobic cultures in rich media, F piliation reached a maximum in the mid-exponential phase. The yield of pili was up to 15 mg 1. In aerated cultures. F pili disappeared in the late exponential phase. In rich media F pili were on average 10-20 μm long; in synthetic media they were an order of magnitude shorter, and less numerous. Addition of metabolic poisons (NaCN, NaN, Na AsO, phenylethyl alcohol) and starving the bacteria caused rapid disappearance of F pili under aerobic conditions, but had no influence on F piliation under anaerobic conditions. Type 1 piliation was not influenced by these drugs or by an alteration of growth conditions.

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/content/journal/micro/10.1099/00221287-130-4-951
1984-04-01
2019-12-14
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