1887

Abstract

The effect of polycationic polypeptides (polylysine, polyarginine and polyhistidine) on the invasion of mammalian cells and plant protoplasts by was studied. In JM cells, a human lymphoblastoid cell line with T cell characteristics, all polycationic polypeptides used increased the invasion rate in a concentration-dependent manner. This effect and the morphological changes revealed by electron microscopy resembled the action of the penetration-enhancing factor previously described by E. Lycke and co-workers. Plant protoplasts of , which are resistant to invasion, showed the same morphological changes in the presence of polycationic polypeptides as observed for JM cells, but were not invaded.

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1984-04-01
2024-12-03
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References

  1. Bannister L. H., Butcher G. A., Mitchell G. H. 1977; Recent advances in understanding the invasion of erythrocytes by merozoites of Plasmodium knowlesi. Bulletin of the World Health Organization 55:163–169
    [Google Scholar]
  2. De Souza W., Souto-Padron T. 1978; Ultrastructural localization of basic proteins on the conoid, rhoptries and micronemes of Toxoplasma gondii. Zeitschrift für Parasitenkunde 56:123–129
    [Google Scholar]
  3. Dulbecco R., Vogt M. 1954; Plaque formation and isolation of pure lines with poliomyelitis viruses. Journal of Experimental Medicine 99:167–199
    [Google Scholar]
  4. Evans P. K. 1976; Higher plant protoplasts: isolation and properties. In Microbial and Plant Protoplasts 1 pp. 51–69 Peberdy J. F., Rose A. H., Rogers H. J., Cocking E. C. Edited by London: Academic Press.;
    [Google Scholar]
  5. Garnham P. C. C., Baker J. R., Bird R. G. 1962; Fine structure of cystic form of Toxoplasma gondii. British Medical Journal 1:83
    [Google Scholar]
  6. Gustafson P. V., Agar H. D., Cramer D. I. 1954; An electron microscope study of Toxoplasma. American Journal of Tropical Medicine 3:1008–1021
    [Google Scholar]
  7. Hartmann W., Galla H. J. 1978; Binding of polylysine to charged bilayer membranes. Molecular organizations of a lipid peptide complex. Biochimica et biophysica acta 509:474–490
    [Google Scholar]
  8. Hughes H. P. A., Van Knapen F. 1982; Characterisation of a secretory antigen from Toxoplasma gondii and its role in circulating antigen production. International Journal of Parasitology 12:433–437
    [Google Scholar]
  9. Kilejian A., Jensen J. B. 1977; A histidine-rich protein from Plasmodium falciparum and its interaction with membranes. Bulletin of the World Health Organization 55:191–197
    [Google Scholar]
  10. Luft J. H. 1961; Improvements in epoxy resin embedding methods. Journal of Biophysical and Biochemical Cytology 9:409
    [Google Scholar]
  11. Lycke E., Carlberg K., Norrby R. 1975; Interactions between Toxoplasma gondii and its host cells: function of the penetration enhancing factor of Toxoplasma. Infection and Immunity 11:853–861
    [Google Scholar]
  12. Mamelak M., Wissig S. L., Bogoroch R., Edelmann I. S. 1969; Physiological and morphological effects of poly-L-lysine on the toad bladder. Journal of Membrane Biology 1:144–176
    [Google Scholar]
  13. Morgan H. C. 1970; A survey of commercially available tissue culture media. In Vitro 6:89–108
    [Google Scholar]
  14. Norrby R. 1970; Host cell penetration of Toxoplasma gondii. Infection and Immunity 2:250–255
    [Google Scholar]
  15. Schneider U., Schwenk H. J., Bornkamm G. 1977; Characterization of EBV-genome negative ‘null’ and ‘T’-cell lines derived from children with acute lymphoblastic leukemic and leukemia transformed non-Hodgkin lymphoma. International Journal of Cancer 19:621–626
    [Google Scholar]
  16. Sheetz M. P., Singer S. J. 1974; Biological membranes as bilayer couples. A molecular mechanism of drug-erythrocyte interactions. Proceedings of the National Academy of Sciences of the United States of America 71:4457–4461
    [Google Scholar]
  17. Werk R. 1982; Attempts to infect plant protoplasts with Toxoplasma gondii. Journal of General Microbiology 128:211–213
    [Google Scholar]
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