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Sporulation of Bacillus sphaericus strain 2297 in a synchronous liquid culture was studied by electron microscopy. The t 0 of sporulation occurred 7 h after the beginning of the lag phase. Crystal-like inclusions first appeared at t 2 and reached their final size between t 5 and t 6. The release of the spore/inclusion complex occurred at about t 15 (22 h after inoculation).
Toxicity against Culex pipiens larvae was related to sporulation and appeared during the early stages of sporulation. The LC50 (24 h) decreased about 105-fold between t 0–2 and t 7, in correlation with the formation of crystalline inclusions. Heat resistance of spores appeared later than toxicity.
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