The Effect of Inhibitors of DNA Repair on the Genetic Instability of Free

Abstract

Various streptomycetes show well defined instabilities that do not appear to be attributable to plasmid loss. The unstable phenotype, in many cases, arises at frequencies too high to be explained by point mutations. The frequency of instability can be enhanced by UV irradiation. Two major repair systems have been found in : the ‘error-free’ system which is inhibited by caffeine and the ‘error-prone’ system which is inhibited by arsenite. Using spores of NRRL 8057 and the virulent actinophage VC11 we have shown that a caffeine inhibitable, host mediated UV repair system is active in spores during early development. Some evidence was also found for the presence of an arsenite inhibitable UV repair system. The caffeine inhibitable UV repair system was found to be involved in the induction of genetic instability in . The arsenite system may be implicated in the repair of such events. Genetic instability was also induced by single strand breaks in DNA caused by P.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-4-887
1984-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/4/mic-130-4-887.html?itemId=/content/journal/micro/10.1099/00221287-130-4-887&mimeType=html&fmt=ahah

References

  1. Flett F., Wotten S. F., Kirby R. 1979; A common host-specificity in the restriction and modification of a bacteriophage by three distinct Streptomyces species. Journal of General Microbiology 110:465–467
    [Google Scholar]
  2. Kirby R., Lewis E. 1981; Unstable genetic elements affecting streptomycin resistance in the streptomycin-producing organisms Streptomyces griseus NCIB 8506 and Streptomyces bikiniensis ISP 5235. Journal of General Microbiology 122:351–355
    [Google Scholar]
  3. Martin J. F., Luengo J. M., Revilla G., Villanueva J. R. 1979; Biochemical genetics of the beta-lactam antibiotic biosynthesis. In 3rd International Symposium on the Genetics of Industrial Microorganisms pp. 83–89 Sebek O. K., Laskin A. I. Edited by Washington, D. C.: American Society for Microbiology.;
    [Google Scholar]
  4. Pogell B. M. 1979; Regulation of aerial mycelium formation in Streptomycetes. In 3rd International Symposium on the Genetics of Industrial Microorganisms pp. 218–224 Sebek O. K., Laskin A. I. Edited by Washington, D. C.: American Society for Microbiology.;
    [Google Scholar]
  5. Rossman T., Meyn M. S., Troll W. 1975; Effect of sodium arsenite on the survival of UV-irradiated Escherichia coli: inhibition of a recA-dependent function. Mutation Research 30:157–162
    [Google Scholar]
  6. Rothman R. H. 1980; Dimer exision in Escherichia coli in the presence of caffeine. Journal of Bacteriology 143:520–524
    [Google Scholar]
  7. Schumann J. P., Jones D. J., Woods D. R. 1982; UV light induction of proteins in Bacteroides fragilisunder anaerobic conditions. Journal of Bacteriology 151:44–47
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-4-887
Loading
/content/journal/micro/10.1099/00221287-130-4-887
Loading

Data & Media loading...

Most cited Most Cited RSS feed