1887

Abstract

The incubation of an enzyme preparation of with UDP-[C]Glc led to the formation of radioactive substances soluble in chloroform/methanol/water (1 : 1 : 0·3, by vol.). These substances had properties similar to the polyprenol diphosphate saccharides. The lipid moiety appears to be an unsaturated polyprenol. Mild acid hydrolysis of the substances liberated five water-soluble products which were separated by TLC and characterized by borohydride reduction-acid hydrolysis, partial acid hydrolysis, methylation analysis, TLC and paper chromatography and paper electrophoresis. The products behaved like: galactose; the disaccharide Glc(1-3)Gal; the tetrasaccharide Glc(1-4)Glc(1-4)Glc(1-3)Gal; an octasaccharide and a pyruvylated octasaccharide. The last two substances were compared by paper electrophoresis, TLC and methylation analysis with an octasaccharide obtained by the action of a hydrolytic enzyme on the exopolysaccharide of . The octasaccharide and the pyruvylated octasaccharide from the lipid behaved like those obtained by enzyme action on the exopolysaccharide. These results suggest that the lipid-bound saccharides are involved in the biosynthesis of the extracellular polysaccharides.

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1984-04-01
2021-08-01
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