1887

Abstract

By varying the composition of the growth medium and the genotype of the bacterial strain, five isoenzymes of CoA-dependent aldehyde dehydrogenase could be detected in . Two isoenzymes (A, mol. wt 520000; and B, mol. wt 370000) were produced only in the presence of ethanolamine and vitamin (or coenzyme) B (‘inducible isoenzyme’), whereas the other three isoenzymes (C, mol. wt 900000; D, mol. wt 120000; and E, mol. wt 720000) were produced only in the absence of ethanolamine and vitamin B (‘repressible isoenzymes’). Partial purification and characterization of these isoenzymes revealed strong similarities, with respect to pH optima and substrate affinities, between isoenzymes within either of the two classes, but significant differences between the two classes. Mutant studies demonstrated that the relationships between the isoenzymes and between CoA-dependent aldehyde dehydrogenase and ethanolamine ammonia-lyase are both structural and regulatory in nature, and a two-operon model is proposed to account for the common control of the enzymes of ethanolamine catabolism in .

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/content/journal/micro/10.1099/00221287-130-4-849
1984-04-01
2021-05-09
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References

  1. Beckwith J. R., Grodzicker T., Arditti R. 1972; Evidence for two sites in the lac promoter region. Journal of Molecular Biology 69:155–160
    [Google Scholar]
  2. Berger D., Weiner H. 1977; Relationship between alcohol preference and biogenic aldehyde metabolizing enzymes in rats. Biochemical Pharmacology 26:841–846
    [Google Scholar]
  3. Blackwell C. M., Turner J. M. 1978; Formation of coenzyme B12-dependent ethanolamine ammonia-lyase and its concerted induction in Escherichia coli. Biochemical Journal 176:751–757
    [Google Scholar]
  4. Burton R. M., Stadtman E. R. 1953; The oxidation of acetaldehyde to acetyl-coenzyme A. Journal of Biological Chemistry 202:873–890
    [Google Scholar]
  5. Clark D. P., Cronan J. E. 1980; Acetaldehyde coenzyme A-dependent dehydrogenase of Escherichia coli. Journal of Bacteriology 144:179–184
    [Google Scholar]
  6. Clark J. F., Jakoby W. B. 1970a; Yeast aldehyde dehydrogenase. III. Preparation of three homogeneous species. Journal of Biological Chemistry 245:6065–6071
    [Google Scholar]
  7. Clark J. F., Jakoby W. B. 1970b; Yeast aldehyde dehydrogenase. IV. Dissociation and re-association of native and hybrid forms. Journal of Biological Chemistry 245:6072–6077
    [Google Scholar]
  8. Dixon M., Webb E. C. 1979 Enzymes, 3rd edn.. London: Longmans;
    [Google Scholar]
  9. Frieden C. 1959; Glutamic dehydrogenase. III. The order of substrate addition in the enzymatic reaction. Journal of Biological Chemistry 234:2891–2896
    [Google Scholar]
  10. Goodman J. I., Tephly T. R. 1972; A comparison of rat and human liver formaldehyde dehydrogenase. Biochimica et hiophysica acta 252:489–505
    [Google Scholar]
  11. Greenfield N. J., Pietruszko R. 1977; Two aldehyde dehydrogenases from human liver: isolation via affinity chromatography and characterization of the isozymes. Biochimica et hiophysica acta 483:35–45
    [Google Scholar]
  12. Jones P. W., Turner J. M. 1984; Interrelationships between the enzymes of ethanolamine metabolism in Escherichia coli. Journal of General Microbiology 130:299–308
    [Google Scholar]
  13. Pennington S. J., Jones P. W., Turner J. M. 1981; The adenosylcobalamin-dependent ethanolamine ammonialyase of Escherichia coli. Biochemical Society Transactions 9:447
    [Google Scholar]
  14. Rudolph F. B., Purich D. L., Fromm H. J. 1968; Coenzyme A-linked aldehyde dehydrogenase from Escherichia coli. I. Partial purification, properties and kinetic studies of the enzyme. Journal of Biological Chemistry 243:5539–5545
    [Google Scholar]
  15. Scarlett F. A., Turner J. M. 1976; Microbial metabolism of amino alcohols. Ethanolamine catabolism mediated by coenzyme B, 2-dependent ethanolamine ammonia-lyase of Escherichia coli and Klebsiella aerogenes. Journal of General Microbiology 95:173–176
    [Google Scholar]
  16. Sedmak J. J., Grossberg S. E. 1977; A rapid, sensitive and versatile assay for protein using Coomassie brilliant blue G250. Analytical Biochemistry 79:544–552
    [Google Scholar]
  17. Shukla S. D., Turner J. M. 1980; Biosynthetic utilization of ethanolamine for lipid synthesis by bacteria. Biochemical Journal 186:13–19
    [Google Scholar]
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