Summary: A strain of sp. (ORS571), isolated from stem nodules on , was grown as a N-fixing continuous culture for up to five weeks (> 80 culture replacements). Under optimum conditions of O supply [< 9 μ dissolved O, 100–160 nmol O (mg dry wt) min], N fixation rates were 300–400 nmol N (mg dry wt) h and nitrogenase activity in culture samples reached 2000 nmol CH (mg dry wt) h, the highest values yet recorded for any species. Other properties of the continuous cultures are described and a comparison is made with previous reports of N fixation by cultures of spp. Significant features were the greater tolerance to O of ORS571 and the retention of fixed N in the cells of this strain.


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