1887

Abstract

Heterocysts of the cyanobacterium occur at the ends of the filaments. These cells, specialized for aerobic N fixation, synchronously differentiate after the fragmentation of filaments grown in a medium free of combined nitrogen. This study has examined the role of transcription during and after heterocyst differentiation. Autoradiography of intact filaments pre-labelled for 3 h with [H]uracil revealed that RNA synthesis occurred at similar rates in vegetative cells, developing (pro)heterocysts and mature heterocysts. Since mature heterocysts no longer divide and contain a similar amount of DNA as vegetative cells, transcription must continue under conditions where replication no longer occurs. Rifampicin, when added to fragmented filaments at concentrations that reduced RNA synthesis by 95% in all cell types, inhibited proheterocyst formation but not the final morphological step of differentiation (pore plug deposition). However, chloramphenicol blocked all stages of heterocyst differentiation. Since the average mRNA half-life was 29 min, and the rifampicin-insensitive stage lasted 3–6 h, the later stages of differentiation may depend on long-lived mRNAs.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-4-789
1984-04-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/4/mic-130-4-789.html?itemId=/content/journal/micro/10.1099/00221287-130-4-789&mimeType=html&fmt=ahah

References

  1. Allen M. D., Arnon D. I. 1955; studies on nitrogen-fixing blue-green algae. I. Growth and nitrogen fixation by Anabaena cylindricaLemm. Plant Physiology 30:336–372
    [Google Scholar]
  2. Fisher R. W., Wolk C. P. 1976; Substance stimulating the differentiation of spores of the blue- green alga Cvlindrospermum. Nature; London: 259394–395
    [Google Scholar]
  3. Fleming H., Haselkorn R. 1974; The program of protein synthesis during differentiation in nitrogen fixing blue-green algae. Cell 3:159–170
    [Google Scholar]
  4. Fogg G. E. 1951; Growth and heterocyst production in Anabaena cylindricaLemm. III. The cytology of heterocysts. Annals of Botany 15:23–35
    [Google Scholar]
  5. Fritsch F. E. 1945 The Structure and Reproduction of Algae II Cambridge, UK:: Cambridge University Press;
    [Google Scholar]
  6. Glaser V. M., Al-Nuri M. A., Groshev V. V., Shestakov S. V. 1973; The labeling of nucleic acids by radioactive precursors in the blue-green algae Anacystis nidulans and Synechocvstis aquatilisSanv. Archiv für Mikrobiologie 92:217–226
    [Google Scholar]
  7. Gray W.J.H., Midgley J.E.M. 1970; The control of ribonucleic acid synthesis in bacteria. Steady state content of messenger ribonucleic acid in Escherichia coli MRE 600. Biochemical Journal 120:279–288
    [Google Scholar]
  8. GROVER I. S., PURI S. 1979; Alteration of heterocyst pattern in Cylindrospermum sp. by rifampicin. Indian Journal of Experimental Biology 17:109–111
    [Google Scholar]
  9. Haselkorn R. 1978; Heterocysts. Annual Review of Plant Physiology 29:319–344
    [Google Scholar]
  10. Janaki S., Wolk C. P. 1982; Synthesis of nitrogenase by isolated heterocysts. Biochimica et biophysica acta 696:187–192
    [Google Scholar]
  11. Ladha J. K., Kumar H. D. 1975; Heterocyst division in two blue-green algae. Archives of Microbiology 102:171–173
    [Google Scholar]
  12. Lang N. J. 1965; Electron microscopic study of heterocyst development in Anabaena azollae Strasburger. Journal of Phycology 1:127–134
    [Google Scholar]
  13. Lang N. J., Fay P. 1971; The heterocysts of blue- green algae. II. Details of ultrastructure. Proceedings of the Royal Society B178:193–203
    [Google Scholar]
  14. Lang N. J., Simon R. D., Wolk C. P. 1972; Correspondence of cyanophycin granules with structured granules in Anabaena cylindrica. Archiv für Mikrobiologie 83:313–320
    [Google Scholar]
  15. Leach C. K., Carr N. G. 1974; In-vitro protein synthesis and measurement of the stability of messenger RNA in the blue-green alga, Anabaena variabilis. Journal of General Microbiology 81:47–58
    [Google Scholar]
  16. Simon R. D. 1973; The effect of chloramphenicol on the production of cyanophycin granule polypeptide in the blue-green alga Anabaena cylindrica. Archiv für Mikrobiologie 92:115–122
    [Google Scholar]
  17. Simon R. D. 1979; Macromolecular composition of heterocysts and spores from Anabaena variabilis. Abstracts of the III International Symposium on Photosynthetic Procaryotes Oxford, England: p. 104 Nichols J. M. Edited by Liverpool, UK:: University of Liverpool;
    [Google Scholar]
  18. Simon R. D. 1980; DNA content of heterocysts and spores of the filamentous cyanobacterium Anabaena variabilis. FEMS Microbiology Letters 8:241–245
    [Google Scholar]
  19. Smith R. J. 1979; Variation in the functional half-life of mRNA in Anacystis nidulans with respect to growth rate. FEMS Microbiology Letters 5:9–11
    [Google Scholar]
  20. Stewart W.D.P., Fitzgerald G. P., Burris R. H. 1968; Acetylene reduction by nitrogen-fixing blue-green algae. Archiv für Mikrobiologie 62:336–348
    [Google Scholar]
  21. Thomas J. 1972; Relationship between age of culture and occurrence of the pigments of photosystem II of photosynthesis in heterocysts of a blue-green alga. Journal of Bacteriology 110:92–95
    [Google Scholar]
  22. Van De Water S., Simon R. D. 1982; Induction and differentiation of heterocysts in the filamentous cyanobacterium Cylindrospermum licheniforme. Journal of General Microbiology 128:917–925
    [Google Scholar]
  23. Wilcox M., Mitchison G. J., Smith R. J. 1973; Pattern formation in the blue-green alga Anabaena.II. Controlled proheterocyst regression. Journal of Cell Science 13:637–649
    [Google Scholar]
  24. Wolk C. P. 1975; Differentiation and pattern formation in filamentous blue-green algae. In Spores VI pp. 85–96 Gerhardt P., Costilow R. N., Sadoff H. L. Edited by Washington, DC:: American Society for Microbiology;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-4-789
Loading
/content/journal/micro/10.1099/00221287-130-4-789
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error