1887

Abstract

Specialized transducing phages and 105, carrying the sporulation gene , were constructed by the prophage transformation method. An RI fragment (2·4 MDal) carrying the gene was isolated from the 105 genome and recloned into the RI site of plasmid pUB110. The recombinant plasmids corrected the sporulation defect of a Spo Rec host, but slightly inhibited the sporulation of a Spo Rec host.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-4-757
1984-04-01
2024-12-13
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/4/mic-130-4-757.html?itemId=/content/journal/micro/10.1099/00221287-130-4-757&mimeType=html&fmt=ahah

References

  1. Hirochika H., Kobayashi Y., Kawamura F., Saito H. 1981; Cloning of sporulation gene spoOB of Bacillus subtilis and its genetic and biochemical analysis. Journal of Bacteriology 146:494–505
    [Google Scholar]
  2. Hirochika H., Kobayashi Y., Kawamura F., Saito H. 1982; Construction and characterization of ɸ105 specialized transducing phages carrying sporulation genes spoOB and spoOF of Bacillus subtilis. Journal of General and Applied Microbiology 28:225–229
    [Google Scholar]
  3. Ikeuchi T., Kudoh , Kurahashi K. 1983; Cloning of sporulation genes spoOA and spoOC of Bacillus subtilis onto pll temperate bacteriophage. Journal of Bacteriology 154:988–991
    [Google Scholar]
  4. Ish-Horowicz D., Burke J. F. 1981; Rapid and efficient cosmid cloning. Nucleic Acids Research 9:2988–2992
    [Google Scholar]
  5. Jenkinson H. F., Mandelstam J. 1983; Cloning of the Bacillus subtilis lys and spoIIIB genes in phage ɸ105. Journal of General Microbiology 129:2229–2240
    [Google Scholar]
  6. Kawamura F., Saito H., Ikeda Y. 1979; A method for construction of specialized transducing phage pll of Bacillus subtilis. Gene 5:87–91
    [Google Scholar]
  7. Kawamura F., Saito H., Hirochika H., Kobayashi Y. 1980; Cloning of sporulation gene, spoOF, in Bacillus subtilis with p11 phage vector. Journal of General and Applied Microbiology 26:345–355
    [Google Scholar]
  8. Kawamura F., Shimotsu H., Saito H., Hirochika H., Kobayashi Y. 1981; Cloning of spoO genes with bacteriophage and plasmid vectors in Bacillus subtilis. In Sporulation and Germination pp. 109–113 Edited by Levinson H. S., Sonenshein A. L., Tipper D. J. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  9. Lovett P. S. 1981; Cloning strategies in Bacillus subtilis. In Sporulation and Germination pp. 40–47 Edited by Levinson H. S., Sonenshein A. L., Tipper D. J. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  10. Piggot P. J., Coote J. G. 1976; Genetic aspects of bacterial endospore formation. Bacteriological Reviews 40:908–962
    [Google Scholar]
  11. Schaeffer P., Ionesco H., Ryter A., Balassa G. 1965; La sporulation de Bacillus subtilis: étude génétique et physiologique. Colloques internationaux du Centre National de la recherche scientifique 124:553–563
    [Google Scholar]
  12. Shimotsu H., Kawamura F., Kobayashi Y., Saito H. 1983; Early sporulation gene spoOF: Nucleotide sequence and analysis of gene product. Proceedings of the National Academy of Sciences of the United States of America 80:658–662
    [Google Scholar]
  13. Sonenshein A. L., Campbell K. M. 1978; Control of gene expression during sporulation. In Spores VII pp. 179–192 Edited by Chambliss G., Vary J. C. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  14. Wilson G. A., Bott K. F. 1968; Nutritional factors influencing the development of competence in the Bacillus subtilis transformation system. Journal of Bacteriology 95:1439–1449
    [Google Scholar]
/content/journal/micro/10.1099/00221287-130-4-757
Loading
/content/journal/micro/10.1099/00221287-130-4-757
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error