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Specialized transducing phages pllspoC and ϕ105spoIIC, carrying the Bacillus subtilis sporulation gene spoIIC, were constructed by the prophage transformation method. An EcoRI fragment (2·4 MDal) carrying the spoIIC gene was isolated from the ϕ105spoIIC genome and recloned into the EcoRI site of plasmid pUB110. The recombinant plasmids corrected the sporulation defect of a Spo− Rec− host, but slightly inhibited the sporulation of a Spo+ Rec− host.
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