RT Journal Article SR Electronic(1) A1 Gardener, Steven A1 Jones, J. GwynfrynYR 1984 T1 A New Solidifying Agent for Culture Media Which Liquefies on Cooling JF Microbiology, VO 130 IS 3 SP 731 OP 733 DO https://doi.org/10.1099/00221287-130-3-731 PB Microbiology Society, SN 1465-2080, AB Pluronic polyol F127, a block copolymer of polypropylene oxide and ethylene oxide, may be used to solidify culture media for the enrichment, isolation and growth of micro-organisms. A stable gel is formed at temperatures above 10 °C, the exact temperature depending on the concentration of polyol used. The most important feature of the polyol gel is that it liquefies as the temperature drops below the critical value for the concentration used. The gel may be auto-claved, and will alternate repeatedly between liquid and gel form. It does not appear to be toxic to aquatic bacteria, is more transparent than agar and may be used to isolate heat-sensitive organisms., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-130-3-731