Pluronic polyol F127, a block copolymer of polypropylene oxide and ethylene oxide, may be used to solidify culture media for the enrichment, isolation and growth of micro-organisms. A stable gel is formed at temperatures above 10 °C, the exact temperature depending on the concentration of polyol used. The most important feature of the polyol gel is that it liquefies as the temperature drops below the critical value for the concentration used. The gel may be auto-claved, and will alternate repeatedly between liquid and gel form. It does not appear to be toxic to aquatic bacteria, is more transparent than agar and may be used to isolate heat-sensitive organisms.


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