1887

Abstract

W3110 cells carrying the ColE3-CA38 plasmid are immune to externally added colicin E8, a newly described member of the E group colicins. By molecular cloning and transposon mutagenesis we localized the colicin E8 immunity gene between the RI site (4·0 kb on the restriction map) and the II site (3·68 kb) of the ColE3-CA38 plasmid. This placed the colicin E8 immunity gene between the colicin E3 immunity gene and , the region which determined mitomycin C sensitivity. Insertion of a transposon into the colicin E3 structural gene prevented the synthesis of active colicin and completely abolished mitomycin C sensitivity, but had no effect on the two immunity genes. In contrast, insertion of a transposon into the colicin E8 immunity gene had no effect upon colicin E3 production or colicin E3 immunity but did abolish mitomycin C sensitivity. The phenotype conferred by plasmids with a transposon inserted into the region of ColE3-CA38 was dependent upon the site of insertion.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-3-701
1984-03-01
2024-11-09
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/3/mic-130-3-701.html?itemId=/content/journal/micro/10.1099/00221287-130-3-701&mimeType=html&fmt=ahah

References

  1. Berg D. E., Weiss A., Crossland L. 1980; Polarity of Tn5 insertion mutation in E. coli . Journal of Bacteriology 142:439–446
    [Google Scholar]
  2. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. Buxton R. S., Drury L. S. 1983; The transposon γδ (TnlOOO) codes for a polypeptide, with an apparent molecular weight of 72000, which is visible in maxi-cells. FEMS Microbiology Letters 7:287–290
    [Google Scholar]
  4. Cooper P. C., James R. 1984; Two new E colicins, E8 and E9, produced by a strain of Escherichia coli . Journal of General Microbiology 130:209–215
    [Google Scholar]
  5. Di Masi R. D., White J. C., Schnaitman C. A., Bradbeer C. 1973; Transport of vitamin B12 in E. coli: cpmmon receptor sites for vitamin B12 and the E colicins on the outer membrane of the cell envelope. Journal of Bacteriology 115:506–573
    [Google Scholar]
  6. Fredericq P. 1949; Sur la resistance croisée entre colicine E et bactériophage II. Comptes rendus des séances de la Société de biologie 143:1011–1013
    [Google Scholar]
  7. Guyer M. S. 1978; The γδ sequence of F is an insertion sequence. Journal of Molecular Biology 126:347–365
    [Google Scholar]
  8. Herschman H. R., Helinski D. R. 1967; Comparative study of the events associated with colicin induction. Journal of Bacteriology 94:691–699
    [Google Scholar]
  9. Hopwood D. A., Kieser T., Wright H. M., Bibb M. J. 1983; Plasmids, recombination and chromosome mapping in Streptomyces lividans 66. Journal of General Microbiology 129:2257–2269
    [Google Scholar]
  10. Jakes K. S., Zinder N. D. 1974; Highly purified colicin E3 contains immunity protein. Proceedings of the National Academy of Sciences of the United States of America 71:3380–3384
    [Google Scholar]
  11. Jorgensen R. A., Rothstein S. J., Reznikoff W. S. 1979; A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance. Molecular and General Genetics 177:65–72
    [Google Scholar]
  12. Kennedy C. K. 1971; Induction of colicin production by high temperature or inhibition of protein synthesis. Journal of Bacteriology 108:10–19
    [Google Scholar]
  13. Males B. M., Stocker B.A.D. 1980; Escherichia coli K317, formerly used to define colicin group E2, produces colicin E7, is immune to colicin E2, and carries a bacteriophage-restricting conjugative plasmid. Journal of Bacteriology 144:524–531
    [Google Scholar]
  14. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Masaki H., Ohta T. 1982; A plasmid region encoding the active fragment and the inhibitor protein of colicin E3-CA38. FEBS Letters 149:129–132
    [Google Scholar]
  16. Miller J. H. 1972 Experiments in Molecular Genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Mock M., Miyada C. G., Gunsalus R. P. 1983; Nucleotide sequence for the catalytic domain of colicin E3 and its immunity protein. Evidence for a third gene overlapping colicin. Nucleic Acids Research 11:3547–3557
    [Google Scholar]
  18. Reeves P. 1965; The adsorption and kinetics of killing by colicin CA 42-E2. Australian Journal of Experimental Biology and Medical Science 43:191–200
    [Google Scholar]
  19. Sabik J. F., Suit J. L., Luria S. E. 1983; cea-kil operon of the ColEl plasmid. Journal of Bacteriology 153:1479–1485
    [Google Scholar]
  20. Shafferman A., Flashner Y., Cohen S. 1979; ColEl DNA sequences interacting in cis, essential for mitomycin C induced lethality. Molecular and General Genetics 176:139–146
    [Google Scholar]
  21. Sidkaro J., Nomura M. 1974; Immunity substance. A protein from E3-colicinogenic cells that accounts for their immunity to colicin E3. Journal of Biological Chemistry 249:445–453
    [Google Scholar]
  22. Sutcliffe J. G. 1978; pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Nucleic Acids Research 5:2721–2727
    [Google Scholar]
  23. Tommassen J., De Geus P., Lugtenberg B. 1982; Regulation of the pho regulon of E. coli K12. Cloning of the regulatory genes phoB and phoR and identification of their gene products. Journal of Molecular Biology 157:265–274
    [Google Scholar]
  24. Vieira J., Messing J. 1982; The pUC plasmids, an M13 mp-7 derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
  25. Watson R., Visentin L. P. 1982; Cloning of the ColE3-CA38 colicin and immunity genes and identification of a plasmid region which enhances colicin production. Gene 19:191–200
    [Google Scholar]
  26. Watson R., Rowsome W., Tsao J., Visentin L. P. 1981; Identification and characterization of Col plasmids from classical colicin E-producing strains. Journal of Bacteriology 147:569–577
    [Google Scholar]
/content/journal/micro/10.1099/00221287-130-3-701
Loading
/content/journal/micro/10.1099/00221287-130-3-701
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error