1887

Abstract

Plasmid D, a hybrid of plasmids P- and R119, mediates polarized chromosome mobilization from one origin in strain PM5006, while the parental plasmids neither individually nor combined mobilize this chromosome. To elucidate its acquired mobilizing ability plasmid D was characterized physically in relation to P- and R119. Restriction patterns of these plasmids were compared and it was shown that D consists of P- and only the r-determinant (r-det) of R119. A mechanism for the formation of plasmid D, via transduction of the r-det and subsequent transposon-like integration into P-, involving insertion sequence IS1, was suggested. Evidence for aberration in plasmid D DNA as a result of r-det integration into P- was attributed to IS1 elements which flank the r-det. Recombination regions of parental plasmid DNA were located on dIII fragments a and of plasmid D and were subsequently inserted into IncP-1 plasmid RP4 that fails to mobilize the chromosome. RP4:: dIII a plasmids did not mobilize the latter chromosome, but rendered the lac. RP4:: dIII plasmids pMC1 and pMC17, containing the fragment in opposite orientations, mobilized the chromosome. For pMC17, mobilization was indistinguishable from that of plasmid D, i.e. having the same orientation and the same single origin. However, mobilization promoted by pMC1 was from two distinctly different origins, different from that of pMC17. This apparently deviates from known examples where inversion of homologous DNA inserted into plasmids leads to mobilization from the same origin but in reverse direction.

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1984-03-01
2024-10-10
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References

  1. Barth P. T. 1979; Plasmid RP4, with Escherichia coli DNA inserted in vitro mediated chromosome transfer. Plasmid 2:130–136
    [Google Scholar]
  2. Barth P. T., Grinter N. J. 1977; Map of plasmid RP4 derived by insertion of transposon C. Journal of Molecular Biology 113:455–474
    [Google Scholar]
  3. Beck Y., Coetzee W. F., Coetzee J. N. 1982; In vitro constructed RP4-prime plasmids mediate orientated mobilization of the Proteus morganii chromosome. Journal of General Microbiology 128:1163–1169
    [Google Scholar]
  4. Blohm D., Goebel W. 1978; Restriction map of the antibiotic resistance plasmid R1drd-19 and its derivatives pKN102 (Rldrd-19B2) and Rldrd-16 for the enzymes BamHI, HindIII, EcoRI and SalI . Molecular and General Genetics 167:119–127
    [Google Scholar]
  5. Chandler M., Silver L., Frey J., Caro L. 1977; suppression of an Escherichia coli dnaA mutation by the integrated R-factor R100.1:generation of small plasmids after integration. Journal of Bacteriology 130:303–311
    [Google Scholar]
  6. Chen C. W., Thomas C. A. 1980; Recovery of DNA segments from agarose gels. Analytical Biochemistry 101:339–341
    [Google Scholar]
  7. Clerget M., Chandler M., Caro L. 1981; The structure of R1drd19: a revised physical map of the plasmid. Molecular and General Genetics 181:183–191
    [Google Scholar]
  8. Clewell D. B., Helinski D. R. 1969; supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an open circular DNA form. Proceedings of the National Academy of Sciences of the United States of America 62:1159–1166
    [Google Scholar]
  9. Coetzee J. N. 1974; Properties of Proteus and Providence strains harbouring recombinant plasmids between P-lac, R1drd19 or R447b. Journal of General Microbiology 80:119–130
    [Google Scholar]
  10. Coetzee J. N. 1975; Chromosome transfer in Protetis mirabilis mediated by a hybrid plasmid. Journal of General Microbiology 86:133–146
    [Google Scholar]
  11. Coetzee J. N. 1978; Extension of a chromosome linkage group of Proteus mirabilis . Journal of General Microbiology 107:155–163
    [Google Scholar]
  12. Coetzee J. N., Jacob A. E., Hedges R. W. 1975; Susceptibility of a hybrid plasmid to excision of genetic material. Molecular and General Genetics 140:7–14
    [Google Scholar]
  13. Coetzee J. N., Van Dijken M. C., Coetzee W. F. 1982; Proteus mirabilis. In Genetic Maps II pp. 152–157 O’Brien S. J. Edited by Bethesda, Maryland: National Cancer Institute, National Institutes of Health;
    [Google Scholar]
  14. Cohen S. N., Miller C. A. 1970; Nonchromosomal antibiotic resistance in bacteria. III. Isolation of the discrete transfer unit of the R-factor R1. Proceedings of the National Academy of Sciences of the United States of America 67:510–516
    [Google Scholar]
  15. Datta N., Hedges R. W., Shaw E. J., Sykes R. B., Richmond M. H. 1971; Properties of an R- factor from Pseudomonas aeruginosa . Journal of Bacteriology 108:1244–1249
    [Google Scholar]
  16. Dénarié J., Rosenberg C., Bergeron B., Boucher C., Michel M., Barate De Bertalmio M. 1977; Potential of RP4:: Mu plasmids for in vivo genetic engineering of Gram-negative bacteria. In DNA Insertion Elements, Plasmids and Episomes pp. 507–520 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Depicker A., Van Montagu M., Schell J. 1977; Physical map of RP4. In DNA Insertion Elements,Plasmids and Episomes pp. 678–679 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Depicker A., De Block M., Inze D., Van Montagu M., Schell J. 1980; IS-like element IS8 in RP4 plasmid and its involvement in cointegration. Gene 10:329–338
    [Google Scholar]
  19. Dressler D., Potter H. 1982; Molecular mechanisms in genetic recombination. Annual Review of Biochemistry 51:727–761
    [Google Scholar]
  20. Eisenstark A. 1977; Genetic recombination in bacteria. Annual Review of Genetics 11:369–396
    [Google Scholar]
  21. Falkow S., Wohlhieter J. A., Citarella R. V., Baron L. S. 1964; Transfer of episomic elements to Proteus. II. Nature of lac + Proteus strains isolated from clinical specimens. Journal of Bacteriology 88:1598–1601
    [Google Scholar]
  22. Grinter N. J. 1981; Analysis of chromosome mobilization using hybrids between plasmid RP4 and a fragment of bacteriophage λ carrying IS1. Plasmid 5:267–276
    [Google Scholar]
  23. Hedges R. W. 1975; R factors from Proteus mirabilis and P. vulgaris . Journal of General Microbiology 87:301–311
    [Google Scholar]
  24. Holloway B. W., Richmond M. H. 1973; R factors used for genetic studies in strains of Pseudomonas aeruginosa and their origin. Genetical Research 21:103–105
    [Google Scholar]
  25. Hu S., Ohtsubo E., Davidson N., Saedler H. 1975; Electron microscope heteroduplex studies of sequence relations among bacterial plasmids: identification and mapping of the insertion sequences IS1 and IS2 in F and R plasmids. Journal of Bacteriology 122:746–755
    [Google Scholar]
  26. Johnson S. R., Romig W. R. 1979; Transposon-facilitated recombination in Vibrio cholerae . Molecular and General Genetics 170:93–101
    [Google Scholar]
  27. Juliot J. S., Boistard P. 1979; Use of RP4-prime plasmids constructed in vitro to promote a polarized transfer of the chromosome in Escherichia coli and Rhizobium meliloti . Molecular and General Genetics 173:289–298
    [Google Scholar]
  28. Kleckner N. 1981; Transposable elements in prokaryotes. Annual Review of Genetics 15:341–404
    [Google Scholar]
  29. Kopecko D. J. 1980; Specialized genetic recombination systems in bacteria: their involvement in gene expression and evolution. Progress in Molecular and Subcellular Biology 7:135–234
    [Google Scholar]
  30. Kopecko D. J., Cohen S. N. 1975; Site-specific-independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci. Proceedings of the National Academy of Sciences of the United States of America 72:1373–1377
    [Google Scholar]
  31. Mcdonell M. W., Simon M. N., Studier F. W. 1977; Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. Journal of Molecular Biology 110:119–146
    [Google Scholar]
  32. Meynell E., Datta N. 1967; Mutant drug resistance factors of high transmissibility. Nature, London 214:885–887
    [Google Scholar]
  33. Nevers R., Saedler H. 1978; Mapping and characterization of an E. coli mutant defective in IS1-mediated deletion formation. Molecular and General Genetics 160:209–214
    [Google Scholar]
  34. Pearce L. E., Mbynell E. 1968; Specific chromosomal affinity of a resistance factor. Journal of General Microbiology 50:159–172
    [Google Scholar]
  35. Perlman D., Rownd R. H. 1975; Transition of R factor NR1 in Proteus mirabilis: molecular structure and replication of NR1 deoxyribonucleic acid. Journal of Bacteriology 123:1013–1034
    [Google Scholar]
  36. Perlman D., Twose T. M., Holland M. J., Rownd R. H. 1975; Denaturation mapping of R factor deoxyribonucleic acid. Journal of Bacteriology 123:1035–1042
    [Google Scholar]
  37. Philippsen P., Kramer R. A., Davis R. W. 1978; Cloning of the yeast ribosomal DNA repeat unit in Sstl and HindlW lambda vectors using genetic and physical size selections. Journal of Molecular Biology 123:371–386
    [Google Scholar]
  38. Plttard A. J., Adelburg E. A. 1963; Gene transfer by F´ strains of Escherichia coli K.12. II. Interaction between F-merogenote and chromosome during transfer. Journal of Bacteriology 85:1402–1408
    [Google Scholar]
  39. Prakash R. K., Sculperoort R. A., Nuti M. P. 1981; Large plasmids of fast-growing rhizobia: homology studies and location of structural nitrogen fixation (nif) genes. Journal of Bacteriology 145:1129–1136
    [Google Scholar]
  40. Ptashne K., Cohen S. N. 1975; Occurrence of insertion sequence (IS) regions on plasmid deoxyribonucleic acid as direct and inverted nucleotide sequence duplications. Journal of Bacteriology 122:776–781
    [Google Scholar]
  41. Reif H.-J., Sadler H. 1977; Chromosomal rearrangements in the gal region of E. coli K12 after integration of IS1. In DNA Insertion Elements, Plasmids and Episomes pp. 81–91 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  42. Rownd R., Micxbl S. 1971; Dissociation and reassociation of RTF and r-determinants of R factor NR1 in Proteus mirabilis . Nature New Biology 234:40–43
    [Google Scholar]
  43. Ruvkun G. B., Ausubbl F. M. 1981; A general method for site-directed mutagenesis in procaryotes. Nature, London 289:85–88
    [Google Scholar]
  44. Saedler H. 1977; IS1 and IS2 in E. coli: Implications for the evolution of the chromosome and some plasmids. In DNA Insertion Elements, Plasmids and Episomes pp. 65–72 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  45. Silver R. P., Fallow S. 1970; Studies on resistance transfer factor deoxyribonucleic acid in Escherichia coli . Journal of Bacteriology 104:340–344
    [Google Scholar]
  46. Stahl F. W. 1979; Special sites in generalized recombination. Annual Review of Genetics 13:7–24
    [Google Scholar]
  47. Starlinger P. 1977; DNA rearrangements in procaryotes. Annual Review in Genetics 11:103–126
    [Google Scholar]
  48. Starlinger P. 1978; In vivo formation of recombinant DNA molecules by IS-elements and transposons. In Genetic Engineering pp. 123–143 Chakrabarty A. M. Edited by West Palm Beach, Florida: C.R.C. Press;
    [Google Scholar]
  49. Starlinger P. 1980; IS-elements and transposons. Plasmid 3:241–259
    [Google Scholar]
  50. Starlinger P., Saedler H. 1976; IS-elements in microorganisms. Current Topics in Microbiology and Immunology 75:111–152
    [Google Scholar]
  51. Van Rensburg A. J. 1970 Studies on Proteus and Providence spheroplasts, l-forms and bacteriophage nucleic acids. M.D. thesis, University of Pretoria, Republic of South Africa
    [Google Scholar]
  52. Wohlhieter J. A., Fallow S., Cltarblla R. V., Baron L. S. 1964; Characterization of DNA from a Proteus strain harboring an episome. Journal of Molecular Biology 9:576–588
    [Google Scholar]
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