RT Journal Article SR Electronic(1) A1 Ros, Joaquim A1 Aguilar, JuanYR 1984 T1 Genetic and Structural Evidence for the Presence of Propanediol Oxidoreductase Isoenzymes in Escherichia coli JF Microbiology, VO 130 IS 3 SP 687 OP 692 DO https://doi.org/10.1099/00221287-130-3-687 PB Microbiology Society, SN 1465-2080, AB The synthesis of propanediol oxidoreductase, an enzyme permitting the anaerobic metabolism of fucose and rhamnose, has been described as being controlled by the prd locus closely linked to the fuc locus in wild-type cells of Escherichia coli. However, strain AA-787, deleted in the fuc and prd loci, grew anaerobically on rhamnose, displaying propanediol oxidoreductase activity. From the deleted strain we derived a constitutive producer of propanediol oxidoreductase able to grow on 1,2-propanediol by oxidizing the diol to lactaldehyde which was further metabolized to lactate. Transduction experiments showed that this ability to use propanediol was closely linked to the rha locus. Peptide mapping of fucose- and rhamnose-induced propanediol oxidoreductase of wild-type cells established structural differences between the two enzymes, indicating two structural genes, one for each sugar metabolizing system., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-130-3-687