Location on the Genome of a Gene Specifying -Acetylserine (Thiol)-lyase Free

Abstract

The plasmid pAB65, derived from a specialized transducing phage carrying DNA from about 52 min on the genome, coded for two polypeptides of approx. 34000. The expression of one was regulated by cyst(e)ine and the gene product and the other by the gene product only. One of these polypeptides was a subunit of -acetylserine (thiol)-lyase (EC 4.2.99.8); the other, associated with the membrane, was the N-terminus of the product of the gene. The pattern of peptide synthesis directed by plasmids carrying smaller DNA fragments indicated that the gene for -acetylserine (thiol)-lyase was transcribed clockwise. The spectrum, amino acid composition and subunit number of the enzyme were determined. The enzyme appears homologous with the gene product. This provides further evidence for the inversion of this region of the genome.

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1984-03-01
2024-03-29
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References

  1. Alexander A. E., Johnson P. 1949 Colloid Science p. 293 Oxford: Oxford University Press;
    [Google Scholar]
  2. Ambler R. P., Scott G. K. 1978; Partial amino acid sequence of penicillinase coded by Escherichia coli plasmid R6K. Proceedings of the National Academy of Sciences of the United States of America 75:3732–3736
    [Google Scholar]
  3. Ashworth J. M., Kornberg H. L. 1966; The anaplerotic fixation of carbon dioxide by Escherichia coli . Proceedings of the Royal Society of London B165:179–188
    [Google Scholar]
  4. Bachmann B. J., Low K. B. 1980; Linkage map of Escherichia coli K12, edition 6. Microbiological Reviews 44:1–56
    [Google Scholar]
  5. Becker M. A., Kredich N. M., Tomkins G. M. 1969; The purification and characterisation of O- acetylserine sulphydrylase-A from Salmonella typhirnurium . Journal of Biological Chemistry 244:2418–2427
    [Google Scholar]
  6. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  7. Bolivar F., Rodriguez R., Greene P. J., Betlach M., Heyneker H. L., Boyer H. W., Crosa J., Falkow S. 1977; II. Construction and characterization of new cloning vehicles, a multipurpose cloning system. Gene 2:95–113
    [Google Scholar]
  8. Boronat A., Jones-Mortimer M. C., Kornberg H. L. 1982; A specialized transducing phage, XpsrlA, for the sorbitol phosphotransferase of Escherichia coli K12. Journal of General Microbiology 128:605–611
    [Google Scholar]
  9. Britton P., Murfitt D., Parra F., Jones-Mortimer M. C., Kornberg H. L. 1982; Phosphotransferase-mediated regulation of carbohydrate utilization in Escherichia coli K12: identification of the products of genes on the specialised transducing phages Xiex (err) and Xgsr (tgs). EMBO Journal 1:907–911
    [Google Scholar]
  10. Britton P., Boronat A., Hartley D. A., Jones-Mortimer M. C., Kornberg H. L., Parra F. 1983; Phosphotransferase-mediated regulation of carbohydrate utilization in Escherichia coli K12: location of the gsr (tgs) and iex (err) genes by specialized transduction. Journal of General Microbiology 129:349–358
    [Google Scholar]
  11. Daoert M., Ehrlich S. D. 1979; Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene 6:23–28
    [Google Scholar]
  12. Davis R. W., Botstein D., Roth J. R. 1980 Advanced Bacterial Genetics Cold Spring Harbor New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Epp C. 1978 Early protein synthesis and its control in bacteriophage lambda Ph.D. thesis University ol Toronto; Canada:
    [Google Scholar]
  14. Fimmel A. L., Loughlin R. E. 1977; Isolation and characterization of cysK mutants of Escherichia coli K12. Journal of General Microbiology 103:37–43
    [Google Scholar]
  15. Henderson P. J. F., Giddbns R. A., Jones-Mortimer M. C. 1976; Transport of galactose, glucose and their molecular analogues by Escherichia coli K12. Biochemical Journal 162:309–320
    [Google Scholar]
  16. Hulanicka M. D., Klopotowski T. 1972; Mutants of Salmonella typhimurium resistant to triazole. Acta biochimica polonica 19:251–260
    [Google Scholar]
  17. Hulanicka M. D., Kredich N. M., Treiman D. M. 1974; The structural gene for O-acetylserine sulphydrylase A in Salmonella typhimurium : identity with the trzA locus. Journal of Biological Chemistry 249:867–872
    [Google Scholar]
  18. Jones-Mortimer M. C. 1968; Positive control of sulphate reduction in Escherichia coli: isolation, characterisation and mapping of cysteineless mutants of E. coli K12. Biochemical Journal 110:589–595
    [Google Scholar]
  19. Kingsman A. J. 1977; The structure of the cys CDHIJ region in unstable auxotrophs of Salmonella typhimurium . Molecular and General Genetics 156:327–332
    [Google Scholar]
  20. Kingsman A. J., Smith D. A. 1978; The nature of genetic instability in auxotrophs of Salmonella typhimurium requiring cysteine or methionine and resistant to inhibition by 1,2,4-triazole. Genetics 89:439–451
    [Google Scholar]
  21. Kingsman A. J., Smith D. A., Hulanicka M. D. 1978; Genetic instability in auxotrophs of Salmonella typhimurium requiring cysteine or methionine and resistant to inhibition by 1,2,4-triazole. Genetics 89:419–437
    [Google Scholar]
  22. Kleckner N., Barker D. F., Ross D. G., Botstein D. 1978; Properties of the translocatable tetracycline-resistance element TnlO in Escherichia coli and bacteriophage lambda. Genetics 90:427–461
    [Google Scholar]
  23. Kredich N. M. 1971; Regulation of L-cysteine biosynthesis in Salmonella typhimurium. I. Effects of growth on varying sulphur sources and 0-acetyl-L- serine on gene expression. Journal of Biological Chemistry 246:3474–3484
    [Google Scholar]
  24. Kredich N. M., Tomkins G. M. 1966; The enzymatic synthesis of L-cysteine in Escherichia coli and Salmonella typhimurium . Journal of Biological Chemistry 241:4955–4965
    [Google Scholar]
  25. Lee J. C., Timasheff S. N. 1974; The calculation of partial specific volumes of proteins in guanidine hydrochloride. Archives of Biochemistry and Biophysics 165:268–273
    [Google Scholar]
  26. Lee L. G., Britton P., Parra F., Boronat A., Kornberg H. L. 1982; Expression of the ptsH+ gene of Escherichia coli cloned on plasmid pBR322: a convenient means for obtaining the histidine-containing carrier protein HPr. FEBS Letters 149:288–292
    [Google Scholar]
  27. Levy S. B. 1970; Resistance of minicells to penicillin lysis: a method of obtaining large quantities of purified minicells. Journal of Bacteriology 103,:836–839
    [Google Scholar]
  28. Parra F., Jones-Mortimer M. C., Kornberg H. L. 1938a; Phosphotransferase-mediated regulation of carbohydrate utilization in Escherichia coli K12: the nature of the iex (err) and gsr (tgs) mutations. Journal of General Microbiology 129:337–348
    [Google Scholar]
  29. Parra F., Britton P., Castle C., Jones-Mortimer M. C., Kornberg H. L. 1983b; Two separate genes involved in sulphate transport in Escherichia coli K12. Journal of General Microbiology 129:357–358
    [Google Scholar]
  30. Perham R. N. 1978; Techniques for determining the amino acid composition and sequence of proteins: Techniques in Protein and Enzyme Biochemistry. Edited by Kornberg H. L., Metcalfe J. C., Northcote D. H., Pogson C. I., Tipton K. F. Techniques in Life Sciences B110 pp. 1–39 Amsterdam &New York: Elsevier;
    [Google Scholar]
  31. Reeve J. 1979; Use of minicells for bacteriophage- directed polypeptide synthesis. Methods in Enzymology 68:493–503
    [Google Scholar]
  32. Sanderson K. E., Hartman P. E. 1978; Linkage map of Salmonella typhimurium, edition V. Microbiological Reviews 42:471–519
    [Google Scholar]
  33. Sanger F., Coulson A. R., Hong G. F., Hill D. F., Petersen G. B. 1982; Nucleotide sequence of bacteriophage X DNA. Journal of Molecular Biology 162:729–773
    [Google Scholar]
  34. Schachman H. K. 1959 Ultracentrifugation in Biochemistry pp. 236–247 New York & London: Academic Press;
    [Google Scholar]
  35. Spencer H. T., Collins J., Monty K. J. 1967; Sequential regulation of cysteine biosynthesis in Salmonella typhimurium . Federation Proceedings 26:677
    [Google Scholar]
  36. Sumner-Smith M., Benchimol S., Murialdo H., Becker A. 1982; The ben gene of bacteriophage X. Mapping, identification and control of synthesis. Journal of Molecular Biology 160:1–22
    [Google Scholar]
  37. Sutcliffe J. G. 1978; Complete nucleotide sequence of Escherichia coli plasmid pBR322. Cold Spring Harbor Symposia on Quantitative Biology 43:77–90
    [Google Scholar]
  38. Szybalski E. H., Szybalski W. 1979; A comprehensive molecular map of bacteriophage lambda. Gene 7:217–270
    [Google Scholar]
  39. Wiater A., Hulanicka M. D. 1979; Properties of cysK mutants of Escherichia coli K12. Acta biochimica polonica 26:21–28
    [Google Scholar]
  40. Wiater A., Filutowicz M., Hulanicka M. D. 1982; A new class of mutants of the cysB regulatory gene for cysteine biosynthesis in Salmonella typhimurium . Journal of General Microbiology 128:1785–1790
    [Google Scholar]
  41. Without B., Boekhout M., Brock M., Kingma J., Van Heerikhuizen H., De Leij L. 1976a; An efficient and reproducible procedure for the for-mation of spheroplasts from variously grown Escherichia coli . Analytical Biochemistry 74:160–170
    [Google Scholar]
  42. Without B., Van Heerikhuizen H., De Leij L. 1976b; How does lysozyme penetrate through the bacterial outer membrane?. Biochimica et biophysica acta 443:534–544
    [Google Scholar]
  43. Worcel A., Burgi E. 1974; Properties of a membrane-attached form of the folded chromosome of Escherichia coli . Journal of Molecular Biology 82:91–105
    [Google Scholar]
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