The plasmid pAB65, derived from a specialized transducing phage carrying DNA from about 52 min on the genome, coded for two polypeptides of approx. 34000. The expression of one was regulated by cyst(e)ine and the gene product and the other by the gene product only. One of these polypeptides was a subunit of -acetylserine (thiol)-lyase (EC; the other, associated with the membrane, was the N-terminus of the product of the gene. The pattern of peptide synthesis directed by plasmids carrying smaller DNA fragments indicated that the gene for -acetylserine (thiol)-lyase was transcribed clockwise. The spectrum, amino acid composition and subunit number of the enzyme were determined. The enzyme appears homologous with the gene product. This provides further evidence for the inversion of this region of the genome.


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