1887

Abstract

We examined bactericidal and opsonizing activity of pooled adult ‘immune’ serum against type b with and without the addition of phagocytes. Four type b strains from cerebrospinal fluid (CSF) and three such strains from the nasopharynx (NP) of healthy children were examined. Duplicate reaction mixtures contained organisms in exponential (E) or stationary phase (S) of growth, serum, a complement source (human agammaglobulinaemic serum), and culture medium (bactericidal assay); separate assays contained the above components and polymorphonuclear leucocytes (opsonization system). A decrease in bacterial density of ⩾ 1 log unit was considered significant. All four S-CSF strains, three of four E-CSF strains and one of three S-NP strains were sensitive to the bactericidal activity of pooled serum. The other E-CSF strain, two S-NP strains and all three E-NP strains were resistant to the bactericidal activity of pooled serum. Two of three E-NP strains were opsonized by pooled serum; the other strains resistant to the bactericidal activity of pooled serum were also resistant to opsonization. Bactericidal and opsonizing activity of serum from an immunized adult was greater than or equal to that of pooled serum against each strain. Assuming normal adults are immune to invasive type b infection, an experimental test reflecting this immunity is the bactericidal activity against CSF isolates tested in stationary phase. We conclude that protection against invasive disease due to type b appears more complex than the presence of bactericidal and opsonizing activity in serum.

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/content/journal/micro/10.1099/00221287-130-3-665
1984-03-01
2021-05-15
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References

  1. Anderson P., Johnston R. B., Smith D. H. 1971; Human serum activities against Hemophilus influenzae, type b. Journal of Clinical Investigation 52:31–38
    [Google Scholar]
  2. Anderson P., Pitt J., Smith D. H. 1976; Synthesis and release of polyribophosphate by Haemophilus influenzae type b in vitro . Infection and Immunity 13:581–389
    [Google Scholar]
  3. Anderson P., Flesher A. L., Shaw S., Harding A. L., Smith D. H. 1980; Phenotypic and genetic variation in the susceptibility of Haemophilus influenzae type b to antibodies to somatic antigens. Journal of Clinical Investigation 65:885–891
    [Google Scholar]
  4. Cohen I. R., Norins L. C. 1968; Antibodies of the IgG, IgM, and IgA classes in newborn and adult sera reactive with Gram-negative bacteria. Journal of Clinical Investigation 47:1053–1062
    [Google Scholar]
  5. Davis S. D., Wedgwood R. J. 1965; Kinetics of the bactericidal action of normal serum on Gramnegative bacteria. Journal of Immunology 95:75–79
    [Google Scholar]
  6. Edberg S., Melton E., Smger J. 1980; Rapid biochemical characterization of Haemophilus species by using the Micro-ID. Journal of Clinical Micro-biology 11:22–26
    [Google Scholar]
  7. Feigin R. D., Richmond D., Hosler M. W., Shackelford P. G. 1971; Reassessment of the role of bactericidal antibody in Hemophilus influen-zae infection. American Journal of the Medical Sciences 262:339–346
    [Google Scholar]
  8. Fothergill L. D., Wright J. 1933; The relation of age incidence to the bactericidal power of blood against the causal organism. Journal of Immunology 24:273–284
    [Google Scholar]
  9. Fothergill L. D., Chandler C., Dingle J. H. 1937; The survival of H. influenzae in phagocytes. Journal of Immunology 32:335–339
    [Google Scholar]
  10. Gitlin D., Rosen F. S., Michael J. G. 1963; Transient 19S gamma1 -globulin deficiency in the newborn infant and its significance. Pediatrics 31:197–208
    [Google Scholar]
  11. Gump D. W., Tarr P., Phillips C. A., Forsyth B. R. 1971; Bactericidal antibodies to Hemophilus influenzae . Proceedings of the Society for Experimental Biology and Medicine 138:76–80
    [Google Scholar]
  12. Howard C. J., Glynn A. A. 1971; The virulence of a strain of Escherichia coli for mice is related to their K antigens conferring resistance to phagocytosis and killing by complement. Immunology 20:767–777
    [Google Scholar]
  13. Jacobs R. F., Wilson C. B., Laxton J. G., Haas J. E., Smith A. L. 1982; Cellular uptake and intracellular activity of antibiotics against Haemophilus influenzae type b. Journal of Infectious Diseases 145:152–159
    [Google Scholar]
  14. Kuo J., Monji N., Swalbe R. S., Mccoy D. W. 1981; A radioactive antigen-binding assay for the measurement of antibody to Haemophilus influenzae type b capsular polysaccharide. Journal of Immunological Methods 43:35–47
    [Google Scholar]
  15. Loeb M. R., Smith D. H. 1980; Outer membrane protein composition in disease isolates of Haemophilus influenzae: pathogenic and epidemiological implications. Infection and Immunity 30:709–717
    [Google Scholar]
  16. Muschel L. H. 1968; Bacterial anatomy and the immune bactericidal reaction. Journal of Immunology 101:819
    [Google Scholar]
  17. Norden C. W. 1970; Variable susceptibility of Haemophilus influenzae meningitis in an adult. A study of bactericidal antibodies and immunoglobulins. New England Journal of Medicine 282:190–194
    [Google Scholar]
  18. Norden C. W. 1972; Variable susceptibility of Haemophilus influenzae type b strains to serum bactericidal activity. Proceedings of the Society for Experimental Biology and Medicine 139:59–61
    [Google Scholar]
  19. Rivers T. M. 1922; Influenzal meningitis. American Journal of Diseases in Children 24:102–124
    [Google Scholar]
  20. Roantrbe R. J. 1976; The relationship of lipopoly- saccharide structure to bacterial virulence. In Microbial Toxins 5 pp. 137 Kadis S., Weinbaum G., Ajl S. J. Edited by New York:: Academic Press.;
    [Google Scholar]
  21. Roantrbb R. J., Pappas N. C. 1960; The survival of strains enteric bacilli in the bloodstream as related their sensitivity to the bactericidal effect of serum. Journal of Clinical Investigation 39:82–88
    [Google Scholar]
  22. Roberts M. C., Smith A. L. 1980; Molecular characterization of ‘plasmid-free’ antibiotic resistant Haemophilus influenzae . Journal of Bacteriology 144:476–479
    [Google Scholar]
  23. Shaw S., Smith A. L., Anderson P., Smith D. 1976; The paradox of Haemophilus influenzae type b bacteraemia in the presence of serum bactericidal activity. Journal of Clinical Investigation 58:1019–1029
    [Google Scholar]
  24. Weller P. F., Smith A. L., Smith D. H., Anderson P. 1978; Rede of immunity in the clearance of bacteraemia due to Haemophilus influenzae . Journal of Infectious Diseases 138:427–436
    [Google Scholar]
  25. Wong K., Roberts M. C., Smith A. L. 1982; The activity of Sch 29482 against type b Haemophilus influenzae lacking or possessing detectable β-lactamase activity. Journal of Antimicrobial Chemistry 9: (suppl. C) 163–170
    [Google Scholar]
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