1887

Abstract

Mutants of that are derepressed for meiosis and spore formation have been isolated and characterized genetically. All are the result of single, recessive nuclear mutations that fall into four linkage groups. Three of these groups are represented by and mutations, which in homozygous diploids confer poor growth and extensive sporulation on a range of non-fermentable media. Haploids carrying any of these mutations are arrested under these conditions in the G1 phase of the cell division cycle as large unbudded cells. The alleles of the mutation complemented all other mutations but were very closely linked to the locus. The fourth linkage group was represented by a mutation conferring temperature-sensitive growth and derepressed sporulation on homozygous diploids grown between 25 °C and 30 °C on media containing galactose or glycerol, but not glucose, as energy source. Above 30 °C this mutant lysed on all media. The mutation it carried failed to complement available mutations. These data bring to five the number of loci at which mutation can lead to derepressed sporulation ( and ). The locus has been mapped 13·9 cM to the left of the centromere on chromosome XV, adjacent to the gene. Diploid strains homozygous for mutations are genetically unstable, giving rise to asporogenous mutants at high frequency, usually as the result of a second mutation unlinked to the mutation. Diploids homozygous for these mutations, and for mutations, show an altered regulation of the formulation of at least three polypeptides normally subject to carbon source repression.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-3-605
1984-03-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/3/mic-130-3-605.html?itemId=/content/journal/micro/10.1099/00221287-130-3-605&mimeType=html&fmt=ahah

References

  1. Broach J. R. 1981; Genes of Saccharomyces cerevisiae. In The Molecular Biology of the Yeast Saccharomyces: Life Cycle and Inheritance pp. 653–727 Strathem J. N., Jones E. W., Broach J. R. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  2. Calvert G. R., Dawes I. W. 1984; Initiation of sporulation in Saccharomyces cerevisiae. Mutations preventing initiation. Journal of General Microbiology 130:615–624
    [Google Scholar]
  3. Dawes I. W. 1975; Study of cell development using derepressed mutations. Nature, London 255:707–708
    [Google Scholar]
  4. Dawes I. W., Hardie I. H. 1974; Selective killing of vegetative cells in sporulated yeast cultures by exposure to diethyl ether. Molecular and General Genetics 131:281–289
    [Google Scholar]
  5. Dawes I. W., Wright J. F., Vezinhet F., Ajam N. 1980; Separation on Urografin gradients of subpopulations from sporulating Saccharomyces cerevisiae cultures. Journal of General Microbiology 119:165–171
    [Google Scholar]
  6. Esposito M. S., Esposito R. E. 1969; The genetic control of sporulation in Saccharomyces cerevisiae. I. The isolation of temperature-sensitive sporulation- deficient mutants. Genetics 61:78–89
    [Google Scholar]
  7. Esposito M. S., Esposito R. E. 1975; Mutants of meiosis and ascospore formation. Methods in Cell Biology 11:303–325
    [Google Scholar]
  8. Esposito R. E., Klapholz S. 1981; Meiosis and ascospore development. In The Molecular Biology of the Yeast Saccharomyces: Life Cycle and Inheritance pp. 211–287 Strathem J. N., Jones E. W., Broach J. R. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  9. Fast D. 1973; Sporulation synchrony in yeast. Journal of Bacteriology 116:925–930
    [Google Scholar]
  10. Hartwell L. H. 1974; Saccharomyces cerevisiae cell cycle. Bacteriological Reviews 38:164–198
    [Google Scholar]
  11. Hawthorne D. C. 1976; UGA mutations and UGA suppressors in yeast. Biochimie 58:179–182
    [Google Scholar]
  12. Henry S. A., Keith A. D. 1971; Membrane properties of saturated fatty acid mutants of yeast revealed by spin labels. Chemistry and Physics of Lipids 7:245–265
    [Google Scholar]
  13. Laemmli V. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. Linnane A. W., Lukins H. B. 1975; Isolation of mitochondria and techniques for studying mitochondrial biogenesis in yeasts. Methods in Cell Biology 12:285–309
    [Google Scholar]
  15. Mortimer R. K., Hawthorne D. C. 1975; Genetic mapping in yeast. Methods in Cell Biology 11:221–234
    [Google Scholar]
  16. Mortimer R. K., Schild D. 1980; Genetic map of Saccharomyces cerevisiae. Microbiological Reviews 44:519–571
    [Google Scholar]
  17. Rothstein R. J., Esposito R. E., Esposito M. S. 1977; The effect of ochre suppression on meiosis and ascospore formation in Saccharomyces. Genetics 85:35–54
    [Google Scholar]
  18. Sherman F. 1969; Use of micromanipulators in yeast studies. Methods in Cell Biology 11:189–200
    [Google Scholar]
  19. Shilo V., Simchen G., Shilo B. 1978; Initiation of meiosis in cell-cycle initiation mutants of Saccharomyces cerevisiae. Experimental Cell Research 112:241–248
    [Google Scholar]
  20. Simchen G. 1974; Are mitotic functions required for meiosis?. Genetics 76:745–753
    [Google Scholar]
  21. Simchen G. 1978; Cell cycle mutants. Annual Review of Genetics 12:161–191
    [Google Scholar]
  22. Vezinhet F., Kinnaird J. H., Dawes I. W. 1979; The physiology of mutants derepressed for sporulation in Saccharomyces cerevisiae. Journal of General Microbiology 115:391–402
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-3-605
Loading
/content/journal/micro/10.1099/00221287-130-3-605
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error